cpg基序重組質(zhì)粒增強小鼠免疫和抗病力效應(yīng)

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1、CpG基序重組質(zhì)粒增強小鼠免疫和抗病力的效應(yīng)遺傳學(xué)專業(yè)研究生：靳艷指導(dǎo)老師：高榮教授為研制安全高效免疫調(diào)節(jié)劑增強動物免疫抗病能力，本實驗設(shè)計合成含11個CpG基序的寡聚核苷酸，重組構(gòu)建含CpG的VRl012質(zhì)粒(VRlC)：制備殼聚糖納米顆粒包裹重組質(zhì)粒(CNP-VRlC)，肌注接種3周齡昆明小白鼠，設(shè)殼聚糖包裹空質(zhì)粒和生理鹽水對照組；接種后28天口服大腸桿菌攻毒觀察小鼠天然免疫的變化和對強毒感染的抵抗力，SandwichELISA測定血清免疫球蛋白和白細(xì)胞介素含量。研究結(jié)果表明：CNP-VRlC能顯著提高小鼠血清IgG、IgM和IgA含量，促進小鼠血清IL一2、I

2、L一6及IL一4顯著增多，同時淋巴細(xì)胞和單核細(xì)胞數(shù)量增加，與對照組小鼠差異顯著(P

3、天然免疫，大腸桿菌攻毒EnhancementoftherecombinantplasmidwithCpGmotifsonimmunityandresistanceofmiceMajor：GeneticsMasterCandiate：JinyanTutor：GaorongInordertodevelopasafeandeffectiveimmunoadjuvanttoenhancetheimmunityandresistanceofanimalsagainstinfection，anovelCpGOligodeoxynucleotidescontaining1CpGm

4、otifswassynthesizedandinsertedintotheVRl012plasmid，designatedasVRIC．ThentherecombinantVR1CwasentrappedwithChitosannanoparticles(CNP)preparedbythemethodofionicCROSSlinkage，andemployedtoinjectmuscularly3-weeksoldKunmingmice；theblankVRl012packedwithCNPandsalinewereusedtoinoculatemiceasthe

5、controlgroups．28daysafterinoculation．a(chǎn)Ilmicewereorallyfed、Ⅳith0．4ml2x108CFU／permousevirulenthemorrhagicenteritisE．colitochallengetheresistanceagainstinfection．SandwichELISAwasusedtodetectthecontentofimmunoglobulinsandifiterleukinsintheseraofmice．Theresultsfoundthatcomparedwithcontrolmi

6、ce，thecontentofIgG’IgaandlgMremarkablyincreasedintheseraofVRlC—CNPtreatedmice(P<0．05)，SOweretheIL一2．IL一4andIL-6．ThenumberoflymphocytesandmonocyteswerealsoraisedconsiderablyinthebloodofVRIC—CNPgroup(P

7、erentdegreesinVRlC-CNPmicewhichweresignificantlyhigherthanthoseofthecontrolgroupsexcepttheneutrophils；andtheVRICtreatedmiceallsurvivedwithoutsymptomsandlesions；whilethecontrolmicemanifestedevidentsymptomsandlesionsWithdiffusehemorrhageinstomach．smallintestineandperitoneum．Thesedemons