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《香菇多糖對(duì)鯉魚(cyprinus carpio)離體培養(yǎng)免疫細(xì)胞的活性調(diào)節(jié)作用》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、農(nóng)業(yè)生物技術(shù)學(xué)報(bào)JournalofAgriculturalBiotechnology2008,16(4):616~621·研究論文·香菇多糖對(duì)鯉魚（）離體培養(yǎng)免疫細(xì)胞的活性調(diào)節(jié)作用*1,?丁煒東1,?張柳2,GalinaJeney?3,?徐跑1,?殷國俊1**曹麗萍（1.中國水產(chǎn)科學(xué)研究院淡水漁業(yè)研究中心，農(nóng)業(yè)部水生動(dòng)物遺傳育種和養(yǎng)殖生物學(xué)重點(diǎn)開放實(shí)驗(yàn)室，無錫214081；2.華中農(nóng)業(yè)大學(xué)，武漢430070；3.ResearchInstituteforFisheries,AquacultureandIrrigation,?Annalight8,SzarvasH4440,H
2、ungary）摘要：采用Percoll密度離心等技術(shù)，對(duì)鯉魚（）的頭腎巨噬細(xì)胞和外周血白細(xì)胞進(jìn)行分離純化，并離體培養(yǎng)。體外暴露不同濃度的香菇多糖后，分別采用MTT(3(4,5dimethylthiazol2yl)2,5diphenyltetrazoliumbromide)法測(cè)定它們對(duì)鯉魚外周血白細(xì)胞增殖的影響；NBT(nitrobluetetrazolium)還原法和Griess試劑顯色法測(cè)定對(duì)頭腎巨噬細(xì)胞的呼吸爆發(fā)的影響；實(shí)時(shí)定量PCR法測(cè)定頭腎巨噬細(xì)胞細(xì)胞因子IL1茁誘導(dǎo)表達(dá)的影響。結(jié)果顯示，香菇多糖1、10和100滋g/mL作用頭腎巨噬細(xì)胞24h后能顯著地誘導(dǎo)巨噬細(xì)
3、胞的氧爆發(fā)活性；作用頭腎巨噬細(xì)胞96h后，其低濃度對(duì)巨噬細(xì)胞生成NO無顯著影響，當(dāng)作用濃度為1000滋g/mL表現(xiàn)出顯著的抑制作用；濃度為100和1000滋g/mL的香菇多糖作用外周血白細(xì)胞24h后顯著地促進(jìn)鯉魚外周血白細(xì)胞的增殖，且在作用巨噬細(xì)胞24h后100滋g/mL能顯著增強(qiáng)IL1茁的體外誘導(dǎo)表達(dá)。香菇多糖對(duì)離體培養(yǎng)鯉魚免疫細(xì)胞有明顯活性作用，對(duì)鯉魚非特異性免疫和特異性免疫具有促進(jìn)作用，是一種有潛力的魚用免疫增強(qiáng)劑。關(guān)鍵詞：香菇多糖；鯉魚；巨噬細(xì)胞；中圖分類號(hào):S185,S188文獻(xiàn)標(biāo)識(shí)碼:A?文章編號(hào):10061304(2008)04061606EffectofL
4、entinanontheActivationofImmunological?CellsCulturedinCAOLiping?1,DINGWeidong?1,ZHANGLiu?2,GalinaJeney?3,XuPao?1,YINGuojun?1**?Theheadkidney(HK)macrophagesandtheperipheralbloodleukocyteswereisolatedfromtheheadkidneyandbloodofrespectivelybypercollgradientdensitycentrifugation,culturedandexp
5、osedtodifferentconcentrationsoflentinan.Toevaluatetheimmunostimulatingeffectsoflentinan,proliferationoftheperipheralbloodleukocytes,respiratoryburstofmacrophageandmRNAexpressionofmacrophageswereinvestigatedbyMTT(3(4,5dimethylthiazol2yl)2,5diphenyl?tetrazoliumbromide),NBT(nitrobluetetrazol
6、ium)reduction,GriessreactionandrealtimePCR,respectively.Resultsshowedthat?proliferationofperipheralbloodleukocytesafter24hincubationbytheinductionoflentinanat100and1000滋g/mLwasmarkedly?stimulated.Lentinanat1,100and1000滋g/mLcouldinducesuperoxideanioninmacrophagesmarkedly.Reductionofnitrico
7、xideofHKmacrophagesafter96hincubationlentinanshowedithadnoconspicuouseffectonnitrogenburstactivityofmacrophages??moreoveritinhibitedthenitrogenburstactivityathigherdoses.TheexpressionofintheHKmacrophagesafter24hstimulationbythepolysaccharideshowedlentinanstimulatede
