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1、5cience曩0■蠶豳2013，39(5)：0940—0945ISSN0257—4799；CN32—1115／SE—mail：CYKE@chin~ourna1．net．on化療藥物環(huán)磷酰胺對家蠶血球密度和精細(xì)胞發(fā)育的影響徐欣郭曉琪崔海波楊洋劉慶信崔為正(山東農(nóng)業(yè)大學(xué)林學(xué)院，山東泰安271018)摘要為探討家蠶作為化療藥物和免疫抑制藥物試驗的模型動物的可能性，研究不同劑量環(huán)磷酰胺對家蠶血球密度、5種血球比率、精母細(xì)胞減數(shù)分裂及精細(xì)胞變形過程的影響。結(jié)果表明，環(huán)磷酰胺能顯著降低家蠶血球密度，抑制血球增殖；對原白血球和顆粒細(xì)胞的比率基本無影響，但使?jié){細(xì)胞比率極顯著升高
2、，使小球細(xì)胞和擬絳色細(xì)胞的比率下降；對精母細(xì)胞減數(shù)分裂和精子變形成熟過程也具有極為顯著的抑制作用。初步確定環(huán)磷酰胺抑制家蠶血球增殖和精細(xì)胞發(fā)育的適宜注射劑量為每克蠶體質(zhì)量200g(即200o,g／g)，適宜添食劑量為500g，高于對哺乳動物的有效劑量。研究結(jié)果證明環(huán)磷酰胺對低等動物家蠶的細(xì)胞分裂和增殖的抑制作用與在高等動物中的作用相同或相似，因此，家蠶有望成為環(huán)磷酰胺類似化療藥物、免疫抑制藥物和不育模型藥物的實驗動物。關(guān)鍵詞家蠶；環(huán)磷酰胺；血球增殖；精細(xì)胞發(fā)育；藥物篩選模型中圖分類號$881．2；R965．1文獻(xiàn)標(biāo)識碼A文章編號0257—4799(2013)05—
3、0940—06EfectsofChemotherapeuticDrugCyclophosphamideonBloodCellDensityandSpermCellDevelopmentofSilkworm，BombyxmoriXUXinGUOXiao—QiCUIHai—BoYANGYangLIUQing—XinCUIWei—Zheng(CollegeofForestry，ShandongAgriculturalUniversity，TaianShandong271018，China)AbstractToinquireintothepossibilityofusin
4、gsilkwormasamodelanimalfortestingchemotherapeuticdrugsandim—munOsuppressiVedrugs，theeffectsofdiferentdosesofcyclophosphamideonthedensityofsilkwormbloodcells．theproportionsof5bloodcorpuscles，spermatocytemeiosisandspermcellformationwereinvestigated．Theresultsshowthatcyclophosphamidecans
5、ignificantlyreducethedensityofsilkwormbloodcelsandinhibitproliferationofbloodcor—puscles．CyclOphOsphamidehasnoefectonproportionsofprohemocytesandgranulocytes，butextremelysignificantlyincreasestheproportionofplasmatocytes．Itreducesproportionsofspherulocytesandoenocytoids，andalsohasex—t
6、remelysignificantinhibitoryeffectonspermatocytemeiosis，spermformationandmaturation．Itispreliminarilydeter—minedthat，toinhibitsilkwormbloodcellproliferationandspermcelldevelopment，theappropriatedoseofcyclophos—phamideviainjectionwas200pgpergramsilkwormbody(i．e．200pg／g)andthatviaoraladm
7、inistrationwas500tJg／g．higherthantheeffectivedosetomammaIs．TheaboveresultsshowedthatcyclophosphamidehasthesameorsimilarroleininhibitingcelldivisionandproliferationbetweenloweranimaIsilkwormandhigheranimals．sosilkwormhasthepotentialtobecometheexperimentalanimalmodelfortestingcyclophosp
8、hamid
