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《鞘內(nèi)注射GDNF對神經(jīng)病理性疼痛大鼠脊髓水平GAD65表達(dá)的影響.pdf》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、中國疼痛醫(yī)學(xué)雜志ChineseJoumalofPainMedicine20l5．21f3doi：10．3969~．issn．1006—9852．2015．03．004鞘內(nèi)注射GDNF對神經(jīng)病理性疼痛大鼠脊髓水平GAD65表達(dá)的影響冰宋歌梁健高園園王存金王紅軍(徐州醫(yī)學(xué)院江蘇省麻醉學(xué)重點(diǎn)實(shí)驗(yàn)室，江蘇省麻醉與鎮(zhèn)痛應(yīng)用技術(shù)重點(diǎn)實(shí)驗(yàn)室，徐州221004徐州市中心醫(yī)院麻醉科，徐州221009)摘要目的：研究神經(jīng)病理性疼痛大鼠脊髓背角谷氨酸脫羧酶65(Glutamatedecarboxylase65，GAD65)的表達(dá)變化及蛛網(wǎng)膜下腔注射膠質(zhì)細(xì)胞源性神經(jīng)營
2、養(yǎng)因子(glialcellline—derivedneurotrophicfactor，GDNF)對其表達(dá)的影響。方法：實(shí)驗(yàn)1：雄性SD大鼠隨機(jī)分為假手術(shù)組(sham組)和坐骨神經(jīng)結(jié)扎組(CCI組)。CCI組又分為術(shù)后ld(D1)、3d(D3)、7d(D7)和14d(D14)組。實(shí)驗(yàn)2：雄性SD大鼠隨機(jī)分為：sham組、D14組、D14+GDNF組和D14+PBS組。兩實(shí)驗(yàn)分別于實(shí)驗(yàn)前及CCI術(shù)后第1、3、7、14d測定熱縮足潛伏期(thermalwithdrawallatency，TwL)及機(jī)械縮足閾值(mechanicalwithdrawa
3、lthreshold，MWT)，處死后取其L4～k脊髓背角，采用Westemblot方法測定GAD65蛋白表達(dá)情況。結(jié)果：與sham組相比，CCI組大鼠TwL及MwT均降低，GAD65表達(dá)水平于術(shù)后3d開始降低，一直持續(xù)到術(shù)后14d；與D14組和D14+PBS組相比，D14+GDNF組大鼠TwL及MWT均升高，GAD65表達(dá)水平升高。結(jié)論：神經(jīng)病理性疼痛的發(fā)生機(jī)制可能和大鼠脊髓背角內(nèi)GAD65表達(dá)降低相關(guān)，而GDNF對其鎮(zhèn)痛作用可能部分是通過增加脊髓GAD65的表達(dá)實(shí)現(xiàn)的。關(guān)鍵詞膠質(zhì)細(xì)胞源性神經(jīng)營養(yǎng)因子；神經(jīng)病理性疼痛；谷氨酸脫羧酶65EI’F
4、ECI’S0FGLIALCELLLINE-DERIVEDNEUROTROPHICFACTORONTHEEXPRESSIONoFGAD65INSPINALCORDINARATMODELOFNEUROPATHICPAIN$SONGGe，LIANGJi，GAOYuan．Yuan，WANGCun．Jin，WANGHong．Jun△(KeyLaboratoryofAnesthesiologyinJiangsuProvince，KeyLaboratoryofAnesthesiaandAnalgesiaApplicationTechnologyinJiang
5、suProvince，XuzhouMedicalCollege，Xuzhou221004；Departmentofanesthesiology,XuzhouCentralHospital，Xuzhou221009)Abstract0bjective：ToinvestigatetheexpressionofGAD65andtheeffectofintrathecalinjectionofglialcellline—derivedneurotrophicfactor(GDNF)ontheexpressionofGAD65inthespinalcor
6、dinaratmodelofneuropathicpain．Methods：Experiment1：MaleSprague—DawleyratswererandomlydividedintoshamoperationgroupandCCIfChronicconstrictiveinjury)group．TheCCIgroupwasfurtherdividedintoldaypostoperativegroup(groupD1)，3dayspostoperativegroup(groupD3)，7dayspostoperativegroup(gr
7、oupD7)and14dayspostoperativegroupfgroupD14)．Experiment2：MaleSprague-Dawleyratswereequallyrandomizedintofourgroups：shamoperationgroup，groupD14，D14combinedwithtreatmentofGDNFgrouprD14+GDNFgroup)andD14combinedwithtreatmentofthePBSgroup(D14+PBSgroup)．Thethermalwithdrawallatency(
8、TWL)andmechanicalwithdrawalthreshold(MWT)weremeasuredbeforetheexperimentand
