番茄抗黃化曲葉病基因ty-5r遺傳分析及分子標(biāo)記

《番茄抗黃化曲葉病基因ty-5r遺傳分析及分子標(biāo)記》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。

1、ClassifiedIndex：Code：10224Confidential(yes／no)：noNO．：120410276DissertationfortheMasterDegreeGeneticAnalysisandGeneMappingofYe／／owLeafCurlVirusResistantGene咖．5‘T0matoin加一5’．01matoCandidate：YangHuanhuanSupervisor：ProfessorLiJingfuDegreeCategory：MasterofAgricultureCollege：CollegeofHorticult

2、ureFirstleveldiscipline：HorticultureSecondleveldiscipline：OlericultureHarbinChinaJune2015萬(wàn)方數(shù)據(jù)目錄㈣㈣㈣㈣刪㈣刪0Y2771679目錄摘要I英文摘要IIIl引言一11．1研究的目的和意義．11．2國(guó)內(nèi)外研究進(jìn)展．11．2．1TYLCV的研究進(jìn)展．11．2．2TYLcv及其傳播一21．2．3番茄黃化曲葉病毒(TYLCV)接種及抗性鑒定21．2．4番茄黃化曲葉病主要防治方法．31．2．5抗番茄黃化曲葉病育種一41．2．6分子標(biāo)記技術(shù)51．2．7抗病品種的選育81．3研究?jī)?nèi)容、技術(shù)路線

3、．92材料與方法112．1試驗(yàn)材料l12．1．1番茄材料l12．1．2試驗(yàn)試劑．112．1．3主要儀器與設(shè)備．112．2．1試驗(yàn)設(shè)計(jì)．112．2．2番茄黃化曲葉病毒病抗性鑒定122．2．3DNA提取及抗感池構(gòu)建132．2．4ty一5基因SSR連鎖群的構(gòu)建及染色體初定位．142．2．5數(shù)據(jù)統(tǒng)計(jì)及連鎖距離的測(cè)定152．2．6番茄黃化曲葉病毒種質(zhì)資源的抗病性鑒定153結(jié)果與分析173．1遺傳規(guī)律分析173．1．1番茄黃化曲葉病抗性遺傳分析173．1．2各世代發(fā)病的株問(wèn)分布173．2基因組DNA的提取183．3SSR分子標(biāo)記結(jié)果】93-3．1SSR引物的篩選一193．3．2抗、

4、感池的構(gòu)建．193-3．3F2代單株的SSR標(biāo)記203．4連鎖關(guān)系作圖20萬(wàn)方數(shù)據(jù)東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文3．4．1SSR標(biāo)記的遺傳連鎖分析．203．4．2連鎖關(guān)系作圖．203．5番茄黃化曲葉病毒病抗病性鑒定．．224討論．．244．1抗病性鑒定244．2抗病基因的分子標(biāo)記244．3番茄抗TvLcv種質(zhì)資源的篩選和品種的選育．255結(jié)論26致謝27參考文獻(xiàn)28附錄36攻讀碩士學(xué)位期間發(fā)表的學(xué)術(shù)論文萬(wàn)方數(shù)據(jù)CONTENrSCoNTENTSAbstractinChineseIAbstractinEnglish。······························

5、···································-··················-···············‘IlI1Introduction······························-·····-····-···-·······-··-···············-···········-····-···-··························。?！??！??！?1．1PurposeandSignificanceofThisResearch··············-‘‘‘11．2Summa

6、ryofDocument’·······-·‘-‘·‘-···········-·······-··‘‘··‘···‘·-‘-··‘·-·····_-···-······-········--······-·······-···--·‘_····‘11．2．1TheResearchofTomatoYellowLeafCurlViruls‘11．2．2IntroductionofTomatoYellowLeafCill"lVirus一21．2．3InoculatingandDentificationofTomatoYellowLeafCurlVirus?！ぁぁぁ····

7、·······t··············-········‘21．2．4PreventionMeasureofTomatoYellowLeafCLIrlVirus···-··。31．2．5ScreeningofResistanceSourcesandInheritanceofResistance··41．2．6MolecularMarkerandResistantbleBreeding-”51．2．7ResistantbleBreeding‘··_······-···············-···············-·····