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1、園藝學(xué)報(bào),2015,42(10):1965–1973.ActaHorticulturaeSinicadoi:10.16420/j.issn.0513-353x.2014-0977;http://www.ahs.ac.cn1965番茄抗黃化曲葉病基因ty-5的分子標(biāo)記及遺傳分析*楊歡歡,許向陽(yáng),姜景彬,劉冠,張賀,李景富(東北農(nóng)業(yè)大學(xué)園藝學(xué)院,哈爾濱150030)摘要:以番茄抗黃化曲葉病毒ty-5基因的抗病材料‘13072’(亞洲蔬菜研究與發(fā)展中心提供)和感病材料‘13493’(早粉2號(hào))為親本配置雜交組合,獲得F1、F2、BC1P1和BC1P24
2、個(gè)世代,對(duì)番茄黃化曲葉病抗性基因ty-5進(jìn)行遺傳規(guī)律分析和基因定位研究。結(jié)果表明,抗性基因ty-5為隱性基因控制。利用657株F2分離單株,應(yīng)用群體分離分析(BSA)法篩選得到與ty-5基因連鎖的5個(gè)多態(tài)性SSR標(biāo)記,構(gòu)建了ty-5基因的分子標(biāo)記連鎖圖譜,將ty-5基因定位到番茄4號(hào)染色體上,物理距離為737kb的區(qū)段內(nèi),兩側(cè)翼標(biāo)記為TES2461和TGS4151,與ty-5遺傳距離分別為2.4cM和3.1cM。生物信息學(xué)分析表明,該區(qū)段存在52個(gè)預(yù)測(cè)候選基因。關(guān)鍵詞:番茄;番茄黃化曲葉?。贿z傳分析;分子標(biāo)記;連鎖圖譜中圖分類號(hào):S641.2文獻(xiàn)
3、標(biāo)志碼:A文章編號(hào):0513-353X(2015)10-1965-09GeneticAnalysisandGeneMappingofTomatoyellowleafcurlvirusResistantGenety-5*YANGHuan-huan,XUXiang-yang,JIANGJing-bin,LIUGuan,ZHANGHe,andLIJing-fu(CollegeofHorticulture,NortheastAgriculturalUniversity,Harbin150030,China)Abstract:Geneticanalysis
4、andgenemappingwerecarriedoutonwhichisresistanttoTomatoyellowleafcurlvirususingtomato(SolanumlycopersicumL.)‘13072’(resistantmaterials)and‘13493’(susceptiblematerials)asexperimentalmaterialsinthisstudy.Theresultsshowedthatarecessivenucleargene,ty-5,controlstheresistancetoTomat
5、oyellowleafcurlvirus.Simplesequencerepeat(SSR)markerswereusedtoconductontheresistant657F2plantsbybulkedsegregantanalysis(BSA)method,andresultedin5SSRmarkerslinkedtothety-5gene.Thety-5genewasmappedtoalinkagegroupcorrespondingtochromosome(Chr.4)oftomato.TheflankingmarkersTES246
6、1andTGS4151werelinkedtothety-5genewithgeneticdistancesof2.4cMand3.1cM,respectively.ThephysicaldistancebetweenmarkersTES2461andTGS4151was737kbbasedonthewholegenomesequenceincluding52candidategenesinthisregion.Keywords:tomato;Tomatoyellowleafcurlvirus;geneticanalysis;SSRmarker;
7、genemapping收稿日期:2015–06–24;修回日期:2015–09–18基金項(xiàng)目:國(guó)家科技支撐計(jì)劃項(xiàng)目(2012BAD02B02-7);國(guó)家自然科學(xué)基金項(xiàng)目(31272171);黑龍江省杰出青年科學(xué)基金項(xiàng)目(JC201204)*通信作者Authorforcorrespondence(E-mail:Lijf_2005@126.com)YangHuan-huan,XuXiang-yang,JiangJing-bin,LiuGuan,ZhangHe,LiJing-fu.GeneticanalysisandgenemappingofTomat
8、oyellowleafcurlvirusresistantgenety-5.1966ActaHorticulturaeSinica,20