資源描述：
《玉米粉質(zhì)胚乳突變體.ppt》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、玉米粉質(zhì)胚乳突變體的研究宋任濤上海大學(xué)玉米是重要的作物和優(yōu)秀的研究模式WalbotandEvans(2003)NatureReviewsGenetics,4:369-379玉米籽粒是“胚乳型”種子的代表玉米籽粒關(guān)鍵發(fā)育時(shí)期SabelliandLarkins,PlantPhysiol149(2009)14-26淀粉粒(S)和蛋白體(P)是主要的儲(chǔ)藏形式籽粒是玉米主要的儲(chǔ)藏器官SSP胚乳是主要的儲(chǔ)藏組織蛋白體（PB）由內(nèi)質(zhì)網(wǎng)（ER）形成HermanandLarkins(1999)PlantCell,11:601-613醇溶蛋
2、白有序地包裝在蛋白體中AdaptedfromLendingandLarkins(1989)PlantCell玉米粉質(zhì)胚乳突變體是早年重要的遺傳突變體FirstreportbySingletonWRandJonesDFintheearlyoflastcentury,includingopaqueendosperm1andopaqueendosperm2.EmersonRA,BeadleGWandFraserAC(1935)Asummaryoflinkagestudiesinmaize.CornellUnivAgricExp
3、StnMemoir180:1-83,described40classicgeneticloci.粉質(zhì)胚乳突變體與高賴氨酸玉米Mertz,ETetal.(1964)Mutantgenethatchangesproteincompositionandincreaseslysinecontentofmaizeendosperm.Science145:279-280(o2).Nelson,OEJretal.(1965)Secondmutantgeneaffectingtheaminoacidpatternofmaizeendos
4、permproteins.Science150:1469-1470(floury2(fl2),o1ando2).Misra,PSetal.(1972)Endospermproteinsynthesisinmaizemutantswithincreasedlysinecontent.Science176:1425-1426(bt2,fl2,o2ando7).玉米已知的粉質(zhì)胚乳突變體Recessiveopaquemutants(o1,o2,o5,o7,o9–o11,o13–o16),Semi-dominantflourymu
5、tants(fl1,fl2andfl3)Dominantmutants(McandDe-B30)BryanC.GibbonandBrianA.Larkins(2005)TrendsGenet21,227-233.已經(jīng)克隆的玉米粉質(zhì)胚乳突變體MutantProductFunctionReferenceo2bZIPtranscriptionfactorDown-regulation22KDazein,LKR,andetc.Schmidt,etal.(1987)Sciencefl222KDa-zeinWithadefectiv
6、eSP,accumulationonERColeman,etal.(1995)PNASDe-B3019KDa-zeinWithaS15PmutationintheSP,un-cleavedKim,etal.(2004)PlantPhysiol.Mc16-kDaγ-zeinAframe-shiftmutation(38-bpdeletion),changedthelast63aminoacidsKim,etal.(2006)PlantJ.fl1AnovelERproteinA-to-Gpointmutation,resul
7、tinginaSer-to-GlysubstitutioninthefirsttransmembranedomainHolding,etal.(2007)PlantCellopaque7和opaque6opaque7(o7)突變體著名的高賴氨酸突變體McWhirter,KS(1971)Aflouryendosperm,highlysinelocusonchromosome10.MNL45:184Misra,PSetal.(1972)Endospermproteinsynthesisinmaizemutantswithin
8、creasedlysinecontent.Science176:1425-1426Burr,FAandBurr,B(1982)ThreemutationsinZeamaysaffectingzeinaccumulation:acomparisonofzeinpolypeptides,invitrosynthesisa
