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《8.護(hù)骨膠囊對(duì)去卵巢大鼠血清骨代謝指標(biāo)的影響-鐘佳賢》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在工程資料-天天文庫。
1、護(hù)骨膠囊對(duì)去卵巢大鼠血清骨代謝指標(biāo)的影響鐘佳賢1,林澤苗1,賈歡歡2,吳玉娥2,陳珺3,曹祺1,李青南2,3,*(1.廣東安諾藥業(yè)股份有限公司,揭陽515300;2.廣東省實(shí)驗(yàn)動(dòng)物重點(diǎn)實(shí)驗(yàn)室,廣東省實(shí)驗(yàn)動(dòng)物監(jiān)測(cè)所,廣州510663;3.廣東藥科大學(xué),生命科學(xué)與生物制藥學(xué)院,廣州510006)[摘要]目的:觀察去卵巢大鼠血清骨代謝指標(biāo)的改變及護(hù)骨膠囊(HG)的調(diào)節(jié)作用。材料方法:采用去卵巢(OVX)模型,分假手術(shù)(Sham),去卵巢(OVX),仙靈骨葆(Pos)和護(hù)骨膠囊低、中、高劑量組(HG-L、HG-M和HG-H)。術(shù)后2天連續(xù)灌胃給藥3個(gè)月,
2、取血清檢測(cè)骨代謝指標(biāo)。結(jié)果:①與Sham組比較,OVX組骨形成指標(biāo)ALP、BALP、UcOC、PINP和骨吸收指標(biāo)CTX均顯著升高,但TRAP5a,TRAPb水平降低;OPG和OPG/RANKL比值顯著降低。②與OVX組比較,HG-L、H組進(jìn)一步升高ALP,HG-H組也升高BALP,HG-L、HG-M組降低BALP;Pos組降低ALP和BALP水平。HG和陽性藥物給藥組降低了UcOC和PINP水平,同時(shí)也降低CTX水平。HG-L組的TRAP5a升高;HG-L、HG-M和Pos組使TRAP5b升高。OPG在HG-H和Pos組增加;OPG/RANKL
3、比值HG和陽性藥物給藥組均升高。結(jié)論:OVX使部分骨形成和骨吸收指標(biāo)增加,表現(xiàn)為骨高轉(zhuǎn)換,HG和仙靈骨葆都能降低這些指標(biāo)。由于HG能在OVX基礎(chǔ)上進(jìn)一步升高ALP,HG-H也升高BALP,而仙靈骨葆沒有進(jìn)一步提高ALP和BALP水平,提示護(hù)骨膠囊刺激成骨的作用較強(qiáng)。[關(guān)鍵詞]中醫(yī)中藥;護(hù)骨膠囊;骨質(zhì)疏松;骨代謝指標(biāo)locatedintheTomb,DongShenJiabang,deferthenextdayfocusedontheassassination.Linping,Zhejiang,1ofwhichliquorwinemasters(W
4、uzhensaidinformationisCarpenter),whogotAfewbayonets,duetomissedfatal,whennightcame17收稿日期:作者簡(jiǎn)介:鐘佳賢(1992-),女,本科生,主要從事藥物研究與推廣,E-mail:853749648@qq.com;*通訊作者:李青南(1956-),女,教授,碩士生導(dǎo)師,主要從事骨質(zhì)疏松模型及相關(guān)藥效學(xué)研究,E-mail:qingnanli@sina.com。EffectsofHugucapsulesonbonemetabolicindicatorsinovariect
5、omizedratsZHONGJia-xian1,LINZe-miao1,JIAHuan-huan2,WUYu-e2,CHENJun3,CAOQi1,CAOKe-guang1,LIQing-nan2,3,*(1.GuangdongAnnolPharmaceuticalCo.,Ltd,Jieyang515300,China;2.GuangdongKeyLaboratoryofLaboratoryAnimals,GuangdongLaboratoryAnimalsMonitoringInstitute,Guangzhou510663,China;3.
6、SchoolofBiosciencesandBiopharmaceutics,GuangdongPharcemeuticalUniversity,Guangzhou510006,China)[Abstract]Objective:TostudytheeffectoftheHugucapsulesonbonemetabolicindicatorsinovariectomizedrats.Method:Ovariectomized(OVX)ratsweredividedintosixgroups,includingShamgroup,OVXgroup
7、,Xianlinggubaogroup(positivecontrol)andHGlow,mediumandhighdosegroup(Sham,OVX,Pos,HG-L,HG-M,HG-H),eachgroupwith10rats.TheratswereigadministratedmedicinecontinuouslyforthreemonthstwodaysafterOVX.TheXianlinggubaoinPoscontrolgroupandnormalsalineinShamandOVXcontrolgroup,respective
8、ly.Aftertreatment,thebonemetabolicindicatorsinserumweredetected.Resu