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1、萬方數(shù)據(jù)解剖科學(xué)進(jìn)展Pro即essofAnatomicMSciences2011Mar,17(2)：164～167人神經(jīng)膠質(zhì)祖細(xì)胞移植于小鼠腦內(nèi)兩側(cè)側(cè)腦室周區(qū)后發(fā)育成人星形膠質(zhì)細(xì)胞韓笑寧1，禹紅梅2，方秀斌”(中國醫(yī)科大學(xué)1．神經(jīng)生物學(xué)教研室，2．附屬第一醫(yī)院神經(jīng)內(nèi)科，遼寧沈陽110004)【摘要】目的觀察人神經(jīng)膠質(zhì)祖細(xì)胞移植于小鼠腦內(nèi)兩側(cè)側(cè)腦室周區(qū)后的形態(tài)結(jié)構(gòu)發(fā)育變化。方法將人神經(jīng)膠質(zhì)祖細(xì)胞移植入出生l天的小鼠腦內(nèi)兩側(cè)側(cè)腦室周區(qū)，用免疫熒光組化技術(shù)和共聚焦顯微鏡觀察移植6個月后，小鼠腦內(nèi)人神經(jīng)膠質(zhì)祖細(xì)胞的分布、
2、分化和形態(tài)結(jié)構(gòu)的特點。結(jié)果人神經(jīng)膠質(zhì)祖細(xì)胞移植6個月后成功地在小鼠腦內(nèi)存活并廣泛分布，主要發(fā)育成人神經(jīng)膠質(zhì)酸性蛋白(glialfihrillaryacidicproteinGFAP)陽性的星形膠質(zhì)細(xì)胞，表現(xiàn)人星形膠質(zhì)細(xì)胞的形態(tài)結(jié)構(gòu)特點，部分神經(jīng)膠質(zhì)祖細(xì)胞分化成硫酸軟骨素蛋白多糖(chondroitinsulphateproteoglycan，NG2)陽性的細(xì)胞。結(jié)論人神經(jīng)膠質(zhì)祖細(xì)胞移植小鼠腦內(nèi)環(huán)境中主要分化為GFAP陽性的星形膠質(zhì)細(xì)胞和部分NG2陽性細(xì)胞。【關(guān)鍵詞】人神經(jīng)膠質(zhì)祖細(xì)胞；星形膠質(zhì)細(xì)胞；小鼠【中圖分類號
3、】R322．81【文獻(xiàn)標(biāo)識碼】A【文章編號】1006-2947(2011)02_0164—04DifferentiationofhumangliaprogenitorceHsintoastrocytesaftertheirtransplantationintobilateralperiareaoflateralventriclesofmousebrainHANXiao—nin91，YUHong—mei2，F(xiàn)ANGXiu—bin‘’(1．DepartmemofNeurobiology，2．DepartmentofN
4、eurologyoftbeFirstAffiliatedHospital，ChinaMedicalUniversity，LiaoningShenyang110004China)【Abstract】objectiveToob8en，ethemophologicaldevelopmentanddifferentiationofhumangilaprogenitorcellsaftertheirtransplantationintobilateralperiareaofthelateralventriclesofmou
5、sebrain．MethodHumangliapmgenitorcellsweretransplantedintobilateralperiareaofthelateralventriclesofmousebrainatfirstpostnatalday(PNDl)，6monthsaftertransplantation，thedistribution,differentiationandmophologicalfeaturesofthehumancellswereobservedbyimmunofluoresc
6、ence‘stainingandconfocalmicroscope．ResultsHumangliapmgenitorcellsweresurvivedanddismbutedwidelyinmousebrain，differentiatedintogliaifibrillaryacidicprotein(GFAP)positiveastrocytesmainlywithmophologiealcharacteristicsofhumanastrocytesandehondroitinsulphateprete
7、oglycan(NG2)positivecells．ConclusionHumangilaprogenitorcellsweredifferentiatedintoastrocytesmainlyandfloraeNG2positivecellsaftertransplantationintomouBebrain．【Keywords】gKaprogenitorcells；astrocytes；mon$e中樞神經(jīng)系統(tǒng)中神經(jīng)膠質(zhì)細(xì)胞主要包括星形膠質(zhì)細(xì)胞，少突膠質(zhì)細(xì)胞和小膠質(zhì)細(xì)胞。在神經(jīng)系統(tǒng)中不但起著支持、營養(yǎng)、保護(hù)的
8、作用，而且還表現(xiàn)調(diào)節(jié)神經(jīng)元生長，神經(jīng)遞質(zhì)傳遞，神經(jīng)元突觸電活動等諸多生物學(xué)功能”1。神經(jīng)膠質(zhì)細(xì)胞功能的異常可以直接導(dǎo)致神經(jīng)系統(tǒng)功能的異常，如脫髓鞘癥狀，癲癇等m引?！臼崭迦掌凇?010-11一11‘通訊作者(Towhomcorrespondenceshouldbeaddressed)神經(jīng)膠質(zhì)細(xì)胞來自于神經(jīng)膠質(zhì)祖細(xì)胞，神經(jīng)膠質(zhì)祖細(xì)胞又由神經(jīng)干細(xì)胞分化而來H。近年來，對某些神
