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1、ForewordTheeraofhigh-speedsequencingandcomputer-amongmicrobesdidnothavetheimpactofthatbasedannotatingsystemshavegeneratedenor-mediatedbetweenamicrobeandaeukaryote.mousdatabasesfromwhichmanynoveldiscov-Thegenomicseraanditsevergrowingdataeriesarebeingmade.Comparativegen
2、omicbasesprovidevastopportunitiestoexplorepo-nucleotideandaminoacidsequenceanalysestentialhorizontalgenetransfersystemsthatmayhaverevealedmanysequencecassettesthatexistbetweenmicrobesandeukaryotesandbe-appearhighlyconserved,thusraisingtheques-tweenlowereukaryotesandhi
3、ghereukaryotes.tionwhetherthesesequenceswereintroducedbyThedirectionofhorizontalgenetransfermaybehorizontalgenetransfermechanisms,orwhetherdominantinonedirection,butmayoccurinthetheywerefortuitousoccurrences.oppositedirection(retrotransfer).ThepriorrecombinantDNAerapr
4、ovidedInreviewingthechaptersofthisbook,Ihavevaluableinsightsontheextentofconservedcometotherealizationthatthediscoveryofhor-geneticmechanismsandbroughttorealizationizontalgenetransferamongdistinctorganismsthatgenescanbemovedacrossspeciesbarriers,ahasincreasedsubstanti
5、allyinthisnewmillen-findingthatwassupported20yearsearlierthanniumanda“new”scientificvocabularyhastherecombinantDNAerabybacterialgeneticex-beenintroduced.Theterms“cross-speciesgeneperiments.Infact,anumberofelegantbacterialtransfer”,“l(fā)ateralgenetransfer”and“horizontalge
6、neticstudieshadimplicatedgenetictransmis-genetransfer”havebeenusedinterchangeably,sionofforeignDNAintobacteriaandintoplants.withthelasttwotermsusedmostfrequently.ThenaturaltransferofplasmidDNAfromToavoidfutureconfusion,theterm“horizontalAgrobacteriumtumefacienstoplant
7、cells,resultinggenetransfer”shouldrepresentthetransferofintheintegrationoftheforeignplasmidDNAgenesacrossdistinctspecies,especiallywhenintothechromosomeoftheplantfollowedbyitsinterkingdomgenetransfertakesplace.Theexpressiontogeneratephenotypicchangeistheterm“l(fā)ateralge
8、netransfer”couldberetainedbestcaseofhorizontalgenetransferthatoccurstoaccommodategenetransferbetweendistinctinnature.Certain