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1、·目錄一主要縮略語英文索引-----------------------------------------------------1二中文摘要--------------------------------------------------------------------3三英文摘要--------------------------------------------------------------------5四論文正文1前言-----------------------------------------------------------------
2、--------72實驗材料------------------------------------------------------------------103實驗方法------------------------------------------------------------------134實驗結(jié)果------------------------------------------------------------------245討論---------------------------------------------------------
3、---------------316結(jié)論------------------------------------------------------------------------34五參考文獻-------------------------------------------------------------------35六文獻綜述-------------------------------------------------------------------39八發(fā)表論文-----------------------------------------
4、--------------------------45九致謝-------------------------------------------------------------------------46····主要縮略語英文索引縮略語全稱中文名稱AA/aaAminoacid氨基酸BPBasepair堿基對BSAAlbuminBovineSerum牛血清白蛋白dNTPDeoxyndeosidetriphosphate脫氧三磷酸核苷酸DTTDithiotheitol二硫蘇糖醇EBEthidiumbromide溴化乙啶EDTAEthylenediamietetraa
5、ceticacid乙二胺四乙酸ELISAEnzymelinkedimmunosorbentassay酶聯(lián)免疫吸附試驗HisHistidine組氨酸6×His6-histidinedomain6個組氨酸殘基IPTGIsoprop-β-D-thiogalactopyranoside異丙基-β-D-硫代半乳糖TPTreponemapallidum梅毒螺旋體KD/kDaKilodalton千道爾頓PBSPhosophatebufferedsaline磷酸鹽緩沖液PCRPolymerasechainreaction聚合酶鏈反應(yīng)SDSSodiumDodecylSulphate十二烷
6、基硫酸鈉SDS-PAGESDS-polyacrylamidegeleletrophroresisSDS-聚丙烯酰胺凝膠電泳TBSTris-bufferedSalineTris緩沖鹽溶液TBSTTween20/Tris-bufferedSaline吐溫20/Tris緩沖鹽溶液TEMEDN,N,N,N,N′N′-四甲基乙胺N′N′-tetramethylethylenediamineTrisTris-(Hydroxymethyl)aminomethane三羥甲基氨基甲烷ECLEnhancedchemiluminescence增強化學發(fā)光GSHReducedglutathio
7、ne還原型谷胱苷肽GSSGOxidizedGlutathione氧化型谷胱甘肽Ni-NTANickelnitrilotriaceticacid鎳-亞硝胺乙酸1····ODOpticaldensity光密度HRPHorseradishperoxidase辣根過氧化物酶r/minRevolutionsperminute每分鐘轉(zhuǎn)數(shù)TBTBmediumTB培養(yǎng)基MrMolecularweight分子量NCNitrocellulose硝酸纖維素膜2····梅毒螺旋體TP0993重組蛋白的表達、純化及免疫活性研究研究生:謝小平導(dǎo)師:吳移謀教授中文摘要