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1、納秒脈沖電場消融乳腺癌的實驗研究［摘要］目的探索納秒脈沖對乳腺癌高轉移細胞株MDA-MB-231的治療效果及其分子機制。方法納秒脈沖電消融乳腺癌細胞及荷瘤小鼠后，檢測接受納秒脈沖治療（實驗組）及未接受納秒脈沖治療（對照組）的小鼠腫瘤細胞增殖、周期和凋亡相關指標，觀察腫瘤的體積、生長形態(tài)及速度等的變化。結果納秒脈沖在體外可以顯著降低細胞活性，誘導細胞凋亡，表現(xiàn)為實驗組CCK-8細胞活性抑制率與對照組比較，差異冇高度統(tǒng)計學意義（P〈0.01）,且各實驗組間差異均有高度統(tǒng)計學意義（P<0.01）；投射電鏡和線粒體電勢實驗觀察到細胞出現(xiàn)凋亡，抑制腫瘤生長。結論納秒脈沖可以通過誘導乳腺癌
2、細胞MDA-MB-231的凋亡而對腫瘤進行消融。［關鍵詞］乳腺癌；MDA-MB-231；納秒脈沖;消融［中圖分類號］R730.5［文獻標識碼］A［文章編號］1673-7210（2015）06（a）-0004-06ExperimentstudyofnanosecondpulsedelectricfieldsablatebreastcancerXIEGuilinlCHENLuyan2CHENXinhua2WENHao3CHENXinmei41.DepartmentofGeneralSurgery,theSecondHospitalofShaoxing,ZhejiangProvinc
3、e,Shaoxing312000,China；2.DepartmentofGeneralSurgery,theFirstAffiliatedHospitalofZhejiangUniversity,ZhejiangProvince,Hangzhou310000,China；1.DepartmentofGeneralSurgery,theFirstAffiliatedHospitalofXinjiangMedicalUniversity,XinjiangUygurAutonomousRegion,Urumqi830054,China；4.SchoolofPharmacy,Shan
4、dongUniversityofTraditionalChineseMedicine,ShandongProvince,Ji'nan250355,China[Abstract]ObjectiveToinvestigatethetreatmenteffectofnanosecondpulsedelectricfields(nsPEFs)onhigh1y-metastatichumanbreastcamcercelllinoMDA-MB-231invitroandinvivotheandthepossiblemolecularmechanism.MethodsAftertreatm
5、entofnsPEFsindifferentparameters,theapoptosis,proliferationandinvasionofMDA-MB-231cellsinratsofnanosecondpulsetherapy(experimentalgroup)andnon—nonosecondpulsetherapy(controlgroup)wereexaminedwithCCK-8,TUNEL,f1owcytometer,transmissionelectronmicroscopy(TEM).Thevolumoftumorsize,grothpatternand
6、speedwereobserved?ResultsInvitro,nsPEFsinhibitedthecellviabilityandinclucedcellapoptosis.ThecellviabilityofCCK-8intheexperimentalgroupwasreducedsignificantlycomparedwithcontrolgroup,thedifferencewasstatisticallysignificant(P<0.01)，andthedifferencesamongdifferentexperimentalgroupwerestatistic
7、allysignificant(P<0.01)；italsopresentedmitochondriadegeneration.FlowcytometershowedthatnsPEFscouldinhibitcancercellproliferation.ConclusionnsPEFscanablatehumanbreastcancerMDA-MB-231byactivatingcaspasesignalingpathway.［Keywords］Breastcancer；MDA-MB-2
