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1、石河子大學(xué)本科畢業(yè)論文王芳海島棉GbMFT2基因在煙草中的遺傳轉(zhuǎn)化本科畢業(yè)論文海島棉GbMFT2基因在煙草中的遺傳轉(zhuǎn)化32石河子大學(xué)本科畢業(yè)論文王芳海島棉GbMFT2基因在煙草中的遺傳轉(zhuǎn)化海島棉GbMFT2基因在煙草中的遺傳轉(zhuǎn)化學(xué)生：王芳指導(dǎo)老師：黃先忠摘要：已經(jīng)證明，被子植物共享一個(gè)保守的磷脂酰乙醇胺結(jié)合（PEBP）結(jié)構(gòu)域來(lái)調(diào)控開花時(shí)間。PEBP家族包括FT-like，TFL1-like和MFT-like三個(gè)亞家族，進(jìn)化分析表明MOTHEROFFTANDTFL1(MFT)是所有PEBP基因的祖先[10]。蛋白質(zhì)序列比
2、對(duì)發(fā)現(xiàn)GbMFT2蛋白的羧基端含有MFT蛋白都含有的脯氨酸，系統(tǒng)進(jìn)化樹分析表明GbMFT2編碼產(chǎn)物與擬南芥AtMFT蛋白親緣關(guān)系較近，他們屬于同一分支。用不同濃度的ABA處理棉花種子后GbMFT2基因表達(dá)變化明顯，表明GbMFT2基因的表達(dá)受ABA的調(diào)節(jié)。為了進(jìn)一步研究該基因的功能，將過(guò)量表達(dá)載體p35S:GbMFT2葉盤法轉(zhuǎn)化煙草，獲得了7株T1代GbMFT2轉(zhuǎn)基因煙草。種植T2代轉(zhuǎn)基因煙草，發(fā)現(xiàn)轉(zhuǎn)基因植株的開花時(shí)間均晚于非轉(zhuǎn)基因植株。并用不同濃度NaCl脅迫處理T2代轉(zhuǎn)GbMFT2基因煙草幼苗，發(fā)現(xiàn)轉(zhuǎn)基因煙草的耐鹽
3、性明顯高于野生型煙草。進(jìn)一步分析GbMFT2基因的功能，以便可以利用該基因調(diào)節(jié)作物的開花時(shí)間及耐鹽性。關(guān)鍵詞：PEBP家族；GbMFT2基因；葉盤法；晚花；耐鹽GeneticTransformationofGossypiumbarbadenseGbMFT2intobaccoAbstract:Angiospermsharingaconservedphosphatidylethanolamine-bindi-ng(PEBP)domainhavebeenshowntobeinvolvedinthecontrolofflowe
4、ringtime.Thefamilyisdividedintothreesubfamilies,FT-like,TFL1-likeandMFT-like.EvolutionanalysisshowedthatMOTHEROFFTANDTFL1(MFT)istheancestralformofallPEBPgenesinplant,GbMFT2genehasaPEBPconservedmotif.InconsistencywithalmostallMFT-likeproteins,GbMFT2hasaconservedp
5、rolineresidueneartheC-terminussimilaritiessimilarwithAtMFTandtheyallbelongtothesameclade.Exogenousabscisicacid(ABA)ofdifferentconcentrationsresultedinsignificantchangeinGbMFT2expressioningerminatingseeds,indicating32石河子大學(xué)本科畢業(yè)論文王芳海島棉GbMFT2基因在煙草中的遺傳轉(zhuǎn)化thattheexpres
6、sionofthisgeneisregulatedbyABA.Tofurtherstudythefunctionofthegene,Itransformedtheover-expressionvectorofp35S:GbMFT2intotobacco.7p35S:GbMFT2transformedtobaccoplantswereacquired.Analysisshowedthatthep35S:GbMFT2transformedtobaccoshowedlatterflowerphenotypethannon-t
7、ransgenicwildtypeplants.Besides,throughsalttoleranceanalysisIfoundthatthep35S:GbMFT2transformedtobaccoshowedstrongersalttolerancethannon-transgenicwildtypeplants.SointhefurtherwecanusetheGbMFT2geneforregulatingthetimeoftheflowersandthesalttoleranceofthecrops.Key
8、word:PEBPfamily;GbMFT2gene;salttolerangce;lateflowering前言金魚草CENTRORADIALIS(CEN）基因的突變?cè)斐烧５臒o(wú)限花序被破壞，莖端形成頂花，克隆研究表明CEN蛋白與動(dòng)物的磷脂酰乙醇胺結(jié)合蛋白(phosphatidylethanolamine-bindingprot
