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1、ENVIRONMENTALMICROBIOLOGY:Bacteria&FungiontheFoodsWeEatTSuzanneSegnerKaren-BeTHg.ScHolTHofheneardailynewsreportsonfood-bornediseaseswereselectedbecausetherehavebeenseveralreportswarn-causedbycontaminatedproduce,dairy,ormeatssuggestsingaboutfood-borneillnessesthatarelinkedtoconsumingtot
2、hepublicthatthesafetyoftheU.S.foodsupplyisinfreshsprouts(Breueretal.,2001;Taorminaetal.,1999).jeopardy.Thesereports,aswellasageneraldistrustinfederalagenciesdueinparttomadcowdiseaseandtoxi-PreparationgenicformsofE.coliingroundbeef,haveresultedinanincreasingdemandfor“organic”foodsandani
3、nterestinStandardmicrobiologyprotocolswereusedforthesustainableagriculture(Macilwain,2004).Itis,therefore,preparationofgrowthmedia.Thedetailsforpreparingmate-timelyandappropriatetoprovideclassroomlecturesandrialsandidentifyingbacteriawerebasedonprotocolspro-laboratoryexperiencestoshowt
4、hatmicrobesareubiqui-videdbytheBacteriologicalAnalyticalManualOnline(www.tousinourenvironment,includinginthefoodsthatwecfsan.fda.gov/~ebam/bam-toc.html)andthesupplieroftheeat(Scholthof,2001;Scholthof,2003).Inpresentingsuchdehydratedculturemedia(CarolinaBiologicalSupplyCo.).material,iti
5、simportantthatwedistinguishbetweencom-Twogrowthmediawereused:nutrientbroth-yeastextractmonmicrobesandpotentiallyharmfulpathogens.Inpar-agar(NBY)andpotatodextroseagar(PDA)supplementedticular,itisimperativetoplaceanemphasisonthefactthatwithtwoantibiotics,ampicillinandstreptomycin(PDA-AS)
6、.manybacteriaandfungiarebothbeneficialandnecessaryNBYwasusedforbacteriabecauseitallowsforgoodgrowthforcropproductionandrobustecosystems.Thishands-onofmostbacteriawithcharacteristiccolonymorphology.PDAlaboratoryexperiencehastheaddedbenefitofreinforcingisacommongrowthmediaforisolatingfun
7、gi.Theadditionsafefoodstorageandpreparation.oftheantibioticsinPDA-ASistoinhibitbacterialgrowth,allowingforbetterdevelopmentofthefungi.ThefungiandThelabdescribedinthisarticlewasspecificallyadoptedbacteriawereculturedat28°Cinastandardlaboratoryincu-foranundergraduatecourse“Pathogens,th
