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《骨髓間充質(zhì)干細(xì)胞靜脈移植對損傷大鼠脊髓vegf、bfgf表達(dá)與血管新生的影響》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。
1、Reaction，RT—PCR)法檢測VEGF和bFGFmRNA表達(dá)變化情況。6．4免疫組化檢測第VIII因子相關(guān)抗原表達(dá)情況6．5采用Basso—Beattie—Bresnahan(BBB)評分和爬網(wǎng)格訓(xùn)練評估大鼠行為學(xué)改變。7．統(tǒng)計學(xué)分析結(jié)果：1．激光共聚焦顯微鏡顯示脊髓損傷后經(jīng)尾靜脈移植的MSCs可達(dá)到脊髓損傷處聚集、存活且可以分泌生長因子VEGF和bFGF。2．RT—PCR結(jié)果顯示VEGF和bFGFmRNA表達(dá)在損傷后比正常增高；移植后1天，MSCs移植組與對照組相比(P>0．05)，無統(tǒng)計學(xué)意義；移植后3天和14天，MSCs移植組均高于同一
2、時間段的對照組且隨時間延長表達(dá)持續(xù)升高。3．第VIII因子相關(guān)抗原免疫組化結(jié)果顯示：移植后1天，MSCs移植組與對照組相比(P>O．05)，無統(tǒng)計學(xué)意義；移植后3天和14天，MSCs移植組微血管密度(microvesseldensity，MVD)均高于同一時間段的對照組且隨時間延長表達(dá)持續(xù)增多。4．BBB評分和爬網(wǎng)格訓(xùn)練顯示移植MSCs可促進(jìn)脊髓損傷大鼠后肢運(yùn)動功能恢復(fù)。結(jié)論：靜脈移植MSCs能在大鼠脊髓損傷區(qū)存活且分泌生長因子VEGF和bFGF，從而促進(jìn)血管新生。關(guān)鍵詞：骨髓間充質(zhì)干細(xì)胞脊髓損傷靜脈移植5Influenceofintravenous
3、administrationofbonemarrowmesenchymalstemcellsonexpressionofVEGF、bFGFandregenerationofbloodvesselafterspinalcordinjuryinratsAbstactObjective：ToobservethesurvivalandmigrationofBoneMarrowMesenchymalStemCel1SlabeledandthechangeonexpressionofVFGFandbfgffactorsatdifferenttimesaftert
4、ransplantionMSCviacaudalvientotreatcontusedspinalcordinjuriedadultSDratsmodel，andtoprovideexperimentalevidenceforthereparingfunctionandmenchanismwhichMSCstreatspinalcordinjury．Methods：1．Ratbonemarrowmesenchymalstemcells(MSCs)fromadultratswereculturedinvitro，theirspecificcellula
5、rsurfacemarkerwereanalysedwithflowcytometer．2．Experimentalanimalandgroups：42healthyadultsrague—dawely(SD)famalrats，meanweigh200—2509，wererandomlydividedinto7groupsincludingnormalcontrolgroup、controlgroupandMSCstransplantationgroups，eachgroups6rats．3．Establishcontusedspinalcordi
6、njuriedSDratmodel：ToadapttheAllen’Simprovedmethod一209weightironbarfallfrom5cmhighandstrikeatT12segmentspinalcordtogetcontusedspinalcordinjuriedratmodels．4．MSCareprelabeledwithCFSEinvitrobeforetransplantation．5．TransplantationofMSCs：InjectMSCsintothespinalcordinjurysiteafterinju
7、ry3daysintransplantgroups，butPBSareinjuriedincontusedcontrol6groups．6．MaterialSandEstimates6．1Heartperfusion、drawthespinalcordfromrat、slice-6．2ThesurviveofCFSE—MSCswhichweretransplantedintospinalcordinjuriedratsviaveinwasobservedbyusingconfocalmicroscopy6．3TheexpressionofVEGFan
8、dbFGFmRNAwasdetectedinspinalcordbyusingRT—PCR．6．4Immun
