資源描述：
《jnk信號(hào)通路對(duì)小膠質(zhì)細(xì)胞功能及其表面tnfr的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、TheinfluencesoftheJNKsignalingpathwayonthefunctionofmicrogialandtheexpressionofTNFRMasterdegreecandidate：ZhangWeiSupervisor：ProfessorLiDanqingMajor：InternalMedicineAbstractobjective：Inthepreviousexperiment，wefoundthatBV2cellspresenteddifferentroleswh
2、enculturedinhypoxiastate．Inthisstudy，wewillfurtherexplortheinfluenceoftheJNKsignalingpathwayonthefunctionofmicrogliaandtheexpressionoftumornecrosisfactorreceptor(TNFR)．Methods：BV2celllineandPC12celllinewereusedtostandformicrogliaandneuronsrespectivel
3、yinthisstudy．BV2cellsaredividedinto：normalcontrolgroup(normoxiagroup)，hypoxialhgroup，hypoxiaIh+SP600125group，hypoxia12hgroupandhypoxia12h+SP600125group．Firstly，westernblotwasusedtodetecttheappropriateSP600125concentrationthatblockedtheJNKsignalingpat
4、hway．Then，BV2cellswereculturedinthesituationofhypoxiawithorwithoutthepresenceofSP600125．PC12cellsurvivalratemeasuredbyCCK一8assayinordertoreflecttheJNKpathwayandBV2cellfunction．AttheSalTletime，analysisBV2cellsTNF—areceptorTNFR1andTNFR2expressionbywest
5、ernblot，anddetectinthedifferentfunctionalstatusBV2cellssecretedexpressionofNGF，TNF-a，andIL-1pbyELISA．Results：1．ThenormalcontrolandtheinjuryofPC12groupsurvivalraterespectivelywas100％and52．37％．TheBV2cellscultureinhypoxiaforlh，thePC12cellsurvivalratewas
6、75．96％；TheSP600125CanenhancePCI2cellsurvivalincreasedto81．88％，significantdifference(P<0．01)．WhileBV2cellhypoxia12h，PCI2cellssurvivalrateis36．87％，theSP600125CanincreasePCI2cellssurvivalrateto57．61％，significantdifference(P<0．01)．2．Undernormalcircumstan
7、ce，theBV2cellsurfacehasnoTNFRlexpression，onlyasmallamountofTNFR2expression．TheBV2cellscultureinhypoxiafor1h，theexpressionofTNFR2increased，addingSP600125canfurtherincreasetheexpressionofTNFR2(P<0．05)；Inhypoxiafor12h，theexpressionofTNFRIincreased，addin
8、g3SP600125canfurtherdecreasetheexpressionofTNFRl(P
