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1、縮略語(yǔ)表英文縮寫英文全稱中文全稱ARFAcuterenalfailure急性腎功能衰竭α-SMAα-smoothmuscleactin平滑肌肌動(dòng)蛋白αC3Complementfactor3補(bǔ)體C3CCK-8Cellcountingkit-8細(xì)胞計(jì)數(shù)試劑盒CKDChronickidneydisease慢性腎臟疾病CRFChronicrenalfailure慢性腎功能衰竭DAB3’3-diaminobenizine二氨基聯(lián)苯胺DAPI4,6-diamidino-2-phenylindole4,6-二脒基-2-苯基吲哚ddH2ODeo
2、ubledistilledwater雙蒸水DMEMDulbecco’smodifiedeagle’smediumDMEM培養(yǎng)液DMSODimethylsulfoxide二甲基亞砜DNADeoxyribonucleicacid脫氧核糖核酸ECMExtracellularmatrix細(xì)胞外基質(zhì)EDTAEthylenediaminetetraaceticacid乙二胺四乙酸ERKExtracellularregulatedprotein細(xì)胞外調(diào)節(jié)蛋白激酶KinasesFBSFetalbovineserum胎牛血清FGF2Basicfi
3、broblastgrowthfactor-2堿性成纖維細(xì)胞生長(zhǎng)因子2FGF23Basicfibroblastgrowthfactor-23堿性成纖維細(xì)胞生長(zhǎng)因子23FGFRFibroblastgrowthfactorreceptor成纖維細(xì)胞生長(zhǎng)因子受體FITCFluoresceinisothiocyanate異硫氰酸熒光素FRS2αFibroblastgrowthfactorreceptor成纖維細(xì)胞生長(zhǎng)因子受體底物2αsubstrate2αFSP-1Fibroblastspecificprotein1成纖維細(xì)胞特異蛋白1HE
4、Hematoxylin-eosin蘇木精-伊紅IgGImmunoglobulinG免疫球蛋白GNRK-49Fnormalratkidney大鼠正常腎成纖維細(xì)胞ODOpticaldensity光密度值PCRPolymeraseChainReaction聚合酶鏈?zhǔn)椒磻?yīng)PBSPhosphatebalancedsolution磷酸鹽緩沖液RNARibonucleicacid核糖核酸ScrSerumcreatinine血清肌酸酐TGF-β1Transforminggrowthfactor-β1轉(zhuǎn)化生長(zhǎng)因子β1UUOUnilateralur
5、eteralobstruction單側(cè)輸尿管梗阻萬(wàn)方數(shù)據(jù)Klothoinhibitsrenaltubulointerstitialfibrosisbyregulatingbasicfibroblastgrowthfactor-2signalingAbstractRenaltubulointerstitialfibrosisisacommonpathwayinchronickidneydisease(CKD)thatleadstoend-stagerenalfailure.Itstwoprominentpathologicalch
6、aracteristicsareexcessivedepositionofextracellularmatrix(ECM)componentsandaccumulationofinterstitialfibroblasts.PreviousstudiessuggestthatthemyofibroblastdifferentiatedfromfibroblastisthemainsourceofECMandthattubuloepithelialcellremodelingcanresultinamyofibroblastphe
7、notypeandthesynthesisofmatrixproteins,suchastypeIandIIIcollagens.Thus,inhibitingfibroblastproliferationanddifferentiationandtubuloepithelialcellremodelingisanimportantwaytoreducethenumberofmyofibroblastsandECMsynthesis,andthestudiesontheunderlyingmechanismscanprovide
8、anovelstrategyandtargetforpreventionandtreatmentofrenalinterstitialfibrosis.Basicfibroblastgrowthfactor-2(FGF2)isinvolvedinprolifer
