資源描述：
《家鴿caveolin-1基因全長cdna的克隆、序列和組織表達(dá)分析》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫。
1、動物學(xué)研究2010，Oct.31(5)：476?482CN53-1040/QISSN0254-5853ZoologicalResearchDOI：10.3724/SP.J.1141.2010.05476家鴿Caveolin-1基因全長cDNA的克隆、序列和組織表達(dá)分析1,2221,22,*張營，郁建鋒，楊麗，王星果，顧志良(1.蘇州大學(xué)基礎(chǔ)醫(yī)學(xué)與生物科學(xué)學(xué)院，江蘇蘇州215123；2.常熟理工學(xué)院生物科學(xué)與工程系，江蘇常熟215500)摘要：窖蛋白(Caveolin)是一類構(gòu)成細(xì)胞膜上胞膜窖主要結(jié)構(gòu)的標(biāo)志蛋白，由Caveolin基因家族編碼而成。Cav
2、eolin-1基因是Caveolin基因家族的成員之一。該實(shí)驗(yàn)采用RT-PCR與RACE(rapidamplificationofcDNAends)技術(shù)成功地克隆了家鴿Caveolin-1基因的全長cDNA。該cDNA全長2605bp，包含537bp的完整編碼區(qū)，編碼178個(gè)氨基酸；分析發(fā)現(xiàn)家鴿Caveolin-1基因編碼區(qū)與牛、家犬、雞、褐家鼠等核苷酸同源性為80.1%～93.4%，氨基酸同源性高達(dá)85.4%～97.2%；半定量RT-PCR分析表明該基因在家鴿各種組織廣泛表達(dá)，脂肪中表達(dá)量最高，各種肌肉中表達(dá)量次之，肝臟中表達(dá)量最低。此結(jié)果說明家鴿C
3、aveolin-1基因可能與脂肪、肌肉中的某些代謝途徑有關(guān)。關(guān)鍵詞：家鴿；窖蛋白基因；RACE；組織表達(dá)分析中圖分類號：Q959.725;Q78;Q951.3;Q341文獻(xiàn)標(biāo)志碼：A文章編號：0254-5853-(2010)05-0476-07CloningandcharacterizationofCaveolin-1geneinpigeon,Columbaliviadomestica1,2221,22,*ZHANGYing,YUJian-Feng,YANGLi，WANGXing-Guo,GUZhi-Liang(1.CollegeofPreclinic
4、alMedicineandBiologicalScience,SoochowUniversity,SuzhouJiangsu215123,China;2.DepartmentofLifeScienceandTechnology,ChangshuInstituteofTechnology,ChangshuJiangsu215500,China)Abstract:Caveolins,aclassofprincipalproteinsformingthestructureofcaveolaeinplasmalemma,wereencodedbycaveoli
5、nsgenefamily.Caveolin-1geneisamemberofcaveolinsgenefamily.Inthepresentstudy,afull-lengthof2605bpcaveolin-1cDNAsequenceinColumbaliviadomestica,whichincludeda537bpcompleteORFencodinga178aminoacidslongputativepeptide,wereobtainedbyusingRT-PCRandRACEtechnique.TheColumbaliviadomestic
6、acaveolin-1CDSshared80.1%?93.4%homologywithBostaurus,Canislupusfamiliaris,GallusgallusandRattusnorvegicus.Meanwhile,theputativeaminoacidsequenceofColumbaliviadomesticacaveolin-1shared85.4%?97.2%homologywiththeabovespecies.Thesemi-quantityRT-PCRrevealedthatCaveolin-1expressionswe
7、redetectableinalltheColumbaliviadomesticatissuesandtheexpressionallevelofcaveolin-1genewashighinadipose,mediuminvariousmuscles,lowinliver.TheseresultsdemonstratedthatCaveolin-1genewaspotentiallyinvolvedinsomemetabolicpathwaysinadiposeandmuscle.Keywords:Columbaliviadomestica;Cave
8、olingene;RapidamplificationofcDNAends;Tissueexp
