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《緩沖液配制方法》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。
1、一．常用緩沖溶液的配制方法1．甘氨酸—鹽酸緩沖液（0.05mol/L）xml0.2mol/L甘氨酸＋yml0.2mol/LHCl，再加水稀釋至200mlpHx/mly/mlpHx/mly/ml2.25044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸Mr=75.07，0.2mol/L甘氨酸溶液含15.01g/L2．甘氨酸—?dú)溲趸c緩沖液（0.05mol/L）xml0.2mol/L甘氨酸＋yml0.2mol/LNaOH
2、，加水稀釋至200mlpHx/mly/mlpHx/mly/ml8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸Mr=75.07，0.2mol/L甘氨酸溶液為15.01g/L3．鄰苯二甲酸—鹽酸緩沖液（0.05mol/L，20℃）xml0.2mol/L鄰苯二甲酸氫鉀＋yml0.2mol/LHCl，再加水稀釋到20mlpHx/mly/mlpHx/mly/ml2.254.
3、6703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.032???鄰苯二甲酸氫鉀Mr=204.23，0.2mol/L鄰苯二甲酸氫鉀溶液含40.85g/L4．檸檬酸—?dú)溲趸c—鹽酸緩沖液10pHNa＋濃度/mol/L檸檬酸/gC6H8O7·H2O氫氧化鈉/gNaOH(97%)鹽酸/mlHCl(濃)最終體積/L*2.20.2021084160103.10.2021083116103.30.2021083106104.
4、30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用時(shí)可以每升中加入1g酚，若最后pH值有變化，再用少量50%氫氧化鈉溶液或濃鹽酸調(diào)節(jié)，置冰箱保存。5．磷酸氫二鈉—檸檬酸緩沖液pH0.2ol/LNa2HPO4/ml0.1mol/L檸檬酸/mlpH0.2mol/LNa2HPO4/ml0.1mol/L檸檬酸/ml2.20.4019.605.210.729.282.41.2418.765.411.158.852.62.18
5、17.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.73
6、1.274.89.8610.147.819.150.855.010.309.708.019.450.5510Na2HPO4Mr=141.98，0.2mol/L溶液為28.40g/L，Na2HPO4·2H2OMr=178.05，0.2mol/L溶液為35.61g/LC6H8O7·H2OMr=210.14，0.1mol/L溶液為21.01g/L6．檸檬酸—檸檬酸鈉緩沖液（0.1mol/L）pH0.1mol/L檸檬酸/ml0.1mol/L檸檬酸鈉/mlpH0.1mol/L檸檬酸/ml0.1mol/L檸檬酸鈉
7、/ml3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8???檸檬酸C6H8O7·H2O，Mr=210.14，0.1mol/L溶液為21.01g/L檸檬酸鈉Na3C6H5O7·
8、2H2O，Mr=294.12，0.1mol/L溶液為29.41g/L7．乙酸—乙酸鈉緩沖液（0.2mol/L，18℃）pH0.2mol/LNaAc/ml0.2mol/LHAc/mlpH0.2mol/LNaAc/ml0.2mol/LHAc/ml3.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.
