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《栽培稻秈粳亞種間遺傳差異及分子標(biāo)記初步研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、varieties，6typicalOryzasativassp．japonica,2cultivarsofOryzasativassp．javanicaand1cultivarsofuplandrice．Theresultindicatedthattherewere23variationalsiteswhichwere5．41％ofentirebasesand8informativesiteswhichwere1．82％ofentirebasesinOryzasatiwThelengthrangeofITSsequenceinOryzasativassp
2、．indicawasrangingfrom425to430bp：thepercentageofG+Ccontentvariedfrom74．25to75．59％．ThelengthrangeofITSsequenceinOryzasativassp．japonicawas425bpandthepercentageofG+Ccontentvariedfrom74．25to75．59％．ThephylogenetictreeconstructedonthebasisoftheITSsequencesshowedthatindicaandjaponicasubs
3、peciesofOryzasativaspeciesformedseparatecladesclearly，meanwhile，Oryzasativassp．jav幼icahavecloserrelationshipswithOryzasativassp．indica．ThestudyshowsthatthesequencesanalysisoftheinternaltranscribedspacerwasagoodtooltodiscriminatebetweensubspeciesofOryzasativaandwassignificantinther
4、esearchofOryzasativaevolution．2．TheanalysisoftherDNAintergenicspacerentiresequencesof13cultivarsinOryzasativa．Lwascarriedoutwith現(xiàn)rufipogonasoutgroup．Inthe13cultivars，therewere6typicalOryzasativassp．japonica,5typicalOryzasativassp．indica,2Oryzasativassp．javanica．Theresultindicatedt
5、hatthelengthrangeofIGSsequenceswasfrom2130to2145bp，thepercentageofG+Ccontentvariedfrom74．59％to75．29％，Vtherewere229variationallociwhichwere10．70％ofentirebasesand76informatirelociwhichwere3．51％ofentirebasesinOryzasativa．38indicaandjaponicaspecificlociwereexistedintheIGSsequencesofOr
6、yzasatJva,inwhichabout34oneswerecoveredaabout400bpsegmentlocatedintheupstreamofIGSsequence．TheindicaandjaponicasubspeciesofOryzasativ馬aswellassomecloselyrelatedcultivars，canbedividedclearlybasedonthephylogenetictreeconstructedbyIGSsequences．Oryzasativassp。javanicahadaclosedrelatio
7、nshipwithjaponicasubspecies．3．Inordertoexploittheutilizationofheterosisbetweenricesubspecies，SSR，ISSR，SRAP，TRAPmarkerswereusedtoanalyzetherelationshipofinter—‘subspeciesandintra—subspeciesinoryzasativa．Theresultindicatedthatall4markerswerehighlycorrelated，and90indica—japonicaspeci
8、ficmarkersthatcouldhelpdifferenti
