資源描述:
《“開放灌注四牽三縫二橫一拉”法大鼠肝移植》由會員上傳分享,免費在線閱讀,更多相關(guān)內(nèi)容在教育資源-天天文庫。
1、“開放灌注四牽三縫二橫一拉”法大鼠原位肝移植術(shù)劉靜基金項目:國家自然科學基金資助項目(NO.39970705)作者單位:363000福建漳州市,175醫(yī)院普外科(劉靜、李冉冉、聶凱、楊愛國);510282廣州市,南方醫(yī)科大學珠江醫(yī)院器官移植科(孫爾維、汪爽、高毅)南方醫(yī)科大學臨床解剖學研究所(鐘世鎮(zhèn))*通訊作者:高毅,電話:020-61643402.E-mail:gaoyi6146@163.com孫爾維2汪爽2李冉冉1聶凱1楊愛國1高毅2鐘世鎮(zhèn)3摘要目的:進一步改進雙袖套法大鼠原位肝移植手術(shù)方法
2、。方法:在Kamada的大鼠原位肝移植已改進方法(方法1)的基礎(chǔ)上,進一步改進方法(方法2)即:供、受體腰背部墊高、腹壁橫切口、彈性拉鉤的牽引、供肝開放灌注、受體術(shù)前用抗菌素、自體血液回輸、肝上下腔靜脈(SVC)的四方向牽引、SVC的“三定點”、“三縫一拉”的縫合方法、小針鉤驅(qū)氣。用方法1及方法2分別進行20次大鼠原位肝移植。結(jié)果:用方法1、方法2(“開放灌注四牽三縫二橫一拉”法)行大鼠原位肝移植的無肝期分別為:16±2min、11±2min,手術(shù)時間分別為:146±22min、94±26min
3、,7天生存率分別為:90%、100%。結(jié)論:方法2行大鼠肝移植,明顯縮短了無肝期、提高移植后大鼠生存率,方法2是一種較好的大鼠原位肝移植的方法。關(guān)鍵詞:大鼠;肝移植;技術(shù)改進Asimpleeffectivemethodforratorthotopiclivertransplantationnamed“pullingonlyonetimeswhilehavingthreestitchesinSVC”.LIUJing﹡,SUNEr-weiWANGShuan,etal.﹡DepartmentofGen
4、eralSurgery,the175thHospitalofPLA,Zhangzhou363000Abstract:ObjectiveToimprovethemethodofratothotopiclivertransplantationusingtwocuffsandshortentheperiodofnonliver,andtoenhancethesuccessrateofoperationandsuvivalrate.MethodsTwomethodswereusedinthecourse
5、ofthisexperiment.ThefirstmethodwasainnovatedmethodfromKamada’smethod.The8/8secondmethodwasainnovatedmethodfromthefirstmethod.Thesecondmethodiscomposedoftechniqesasfollowing:Rats’back-waistisraisedwithspacer;Elasticclawsfordragginghypochondriumandlate
6、ralincisionsonrats’abdomenwallareused;Portalveinarenotligatingtotheductwhichisusedforinstillatingdonor’slivers;Recipientsareinjectedpenicillinbeforeoperationforrecipientsbegintobecarriedoutandareself-transfused;SVCispulledtotheleft,theright,theheadan
7、dthetail;SuturesilkispulledonlyonetimesperhavingthreestitchesinSVC;SVCisfixedinthreepoints;Gasisdrivenoutofveinsusingabentneedleconnectinginjector.Ratorthotopiclivertransplantationwascarriedoutin20casesbythefirstmethodandother20casesbythesecondmethod
8、.ResultsThetimeofnonliverwas16±2min、11±2minbythefirstmethod、thesecondmethodrespectively.Thetimeofoperationwas146±22min、94±26minrespectively.Survivalratesof7daysare90%、100%respectively.ConclusionsThesecondmetheodcansimplifytheoperation’sprocedure,shor