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《力竭運動與鈍挫傷對大鼠骨骼肌衛(wèi)星細(xì)胞與肝細(xì)胞生長因子的影響》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、葉雷雷：力竭運動及鈍挫傷對人鼠骨日hi口t肌比／Zj3二!刪H岜以盯"細(xì)胞生K岡子的影響111ABSTRACTObjective：Thepurposeofthispaperwastoinvestigatedeffectsofone．timeexhaustiveexerciseandcontusiononskeletalmusclesatellitecellsandhepatocytegrowthfactor(HGF)inrat．Methods：24malewistarratsof6一week．oldwererandoml5，dividedinto4gr
2、oups：controlgroup(C)?andimmediatelyafterexhaustiveexercisegroup(Exhaustiive，EO)，24hgroup(E24)，48hgroup(E48)．Theone-timeexhaustiveexercisegroupperformedanexhaustivedownhillrunningtoexhaustionstate．Theexhaustiveexercisegroupandthecontrolgroupwereanesthetizedwith20％urethanebyanintr
3、aperitonealinjectionatdifferenttimepointsafterthelastexhaustiveexerciseandinaquietstate．5mlbloodssamplewastakenfromtheabdominalaortatomakeserum，andtheserumwasusedtomeasurethehepatocytegrowthfactorcontents．Asideoftherectusfemorismusclesamplewasfixedwith2．5％glutaraldehydeforproduc
4、ingultrathinsectionsusedtoobservetheultrastructuralchangesbytransmissionelectronmicroscope。TheothersidewasreservedinliquidnitrogentomeasurethehepatocytegrovnhfactorcontentsAnother4adultratswasdividedinto4groups：controlgroup，Immediatelyaftercontusiongroup(Contusion．DO)。24hgroup(D
5、24)，48hgroup(D48)．Atdifferenttimepointsaftercontusion，asideoftherectusfemorismusclesampleswerefixedwith2．5％glutaraldehydeforproducingultrathinsectionsusedtoobservetheultrastructuralchangesbytransmissionelectronmicroscope．Theothersideofthevastuslateralismuscleforcultureofsatellit
6、ecells．Anotherneonatalrat‘SvastuslateralisalsousedforcultureofmusclesatellitecellsResults：1)Afterofle—timeexhaustiveexercisejexhaustiveexercisegroup‘s(E0，E24，E48)skeletalmuscleultrastructuralstructurewassiginificiantlychanged：@Themyofibrilbecamethinnerandmoreincompact．HbandandIb
7、andtookill—defined．MlinesandZlinesblurred．Zlineswerefloweddistortedanddisappeared；@Musclegapwidenedwithaninterstitialedema；@Endoplasmicreticulumslightlydeformed．oreventwisted；@Somematrixbecomeshallow，andmitochondrialswereobviouslydeformed，distortedandevenswollen．Withtimeextendin
8、g，thedamageoftheexhaustiveexercisegroup’Smuscle
