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1、維普資訊http://www.cqvip.com第17卷第2期激光生物學(xué)報(bào)V01．17No．22008年4月ACTALASERBIOLOGYSINICAApr．2008·基礎(chǔ)研究·不同氮源對(duì)蛋白核小球藻生長(zhǎng)色素和中性脂肪積累的影響、王順昌，王陶，趙世光，吳躍進(jìn)，余增亮(1．中國科學(xué)院等離子體物理研究所離子束生物工程重點(diǎn)實(shí)驗(yàn)室，安徽合肥230031；2．淮南師范學(xué)院化學(xué)生物系，安徽淮南232001)摘要：?jiǎn)渭?xì)胞微藻在生長(zhǎng)發(fā)育過程中，所積累的中性脂肪具有潛在的生物燃料價(jià)值。不同氮源對(duì)藻類的生長(zhǎng)具有顯著影響。研究
2、了氨態(tài)氮、尿素氮和硝酸態(tài)氮對(duì)蛋白核小球藻生長(zhǎng)、色素和中性脂肪積累的影響。結(jié)果顯示，不同氮源對(duì)培養(yǎng)液pH有顯著影響，以氨態(tài)氮為氮源導(dǎo)致培養(yǎng)液pH降低，而硝酸態(tài)氮導(dǎo)致培養(yǎng)液pH升高，培養(yǎng)液pH的波動(dòng)可被添加的Hepes所穩(wěn)定，并促進(jìn)以氨態(tài)氮為氮源的蛋白核小球藻的生長(zhǎng)。尿素氮對(duì)蛋白核小球藻生長(zhǎng)、色素積累的效果優(yōu)于氨態(tài)氮和硝酸態(tài)氮，硝酸態(tài)氮在中性脂肪積累上優(yōu)于尿素氮和氨態(tài)氮，添加Hepes對(duì)氮饑餓后蛋白核小球藻的中性脂肪積累無顯著影響。關(guān)鍵詞：蛋白核小球藻；氮源；色素積累；中性脂肪中圖分類號(hào)：$963文獻(xiàn)標(biāo)識(shí)碼：A
3、文章編號(hào)：1007-7146(2008)02-0197-05Growth，PigmentandNeutralLipidsAccumulationinChlorellapyrenoidosaunderDiferentNitrogenousSourcesWANGShun．chang一，WANGTao，ZHAOShi．guang，UYue-jin，YUZeng．1iang(1．KeyLaborotaryofIonBeamBiotechnology，InstituteofPlasmaPhysics，ChineseA
4、cademyofScience，Anhui23003，Hefei，China；2．DepartmentofChemistryandBiology，HuainanNormalUniversity，Huainan232001，Anhui，China)Abstract：Theunicellularmicroalgaeaccumulatedtheneutrallipids，whichcouldbepotentiallyusedasthebio—fuels．Thegrowthofalgaewasaffectedbym
5、anyenvironmentalfactorssuchaspHandnitrogenoussources．Inordertoinvesti—gatetheeffectsofdifferentnitrogenoussourcesonthegrowth，pigmentsandneutrallipidsaccumulationonalgae，thegreenalgaeChloreUapyrenoidosawascultivatedinammonia，ureaandnitrateasthenitrogenousso
6、urces，respectively．Theresultspresentedheredemonstratedthatthegrowthproperties，pigmentsandneutralaccumulationofC．pyrenoidosawereaffectedbvdifferentsourcesofammonia．ureaandnitrate．TheresultsindicatedthatthemediumpHwasreducedbytheusingofammoniaasthenitrogenou
7、ssource，whilethenitratecausedthemediumpHelevated．ThefluctuationofthemediumpHcouldbebalancedbytheadditionofHepesbuffer．Theureahasbettereffectsonpromotinggrowthandpiginentsacculnulationthanthatofammoniaandnitrate，whereasthenitratehasbettereffectsonneutrallip
8、idsaccumu—lationthanthatofammoniaandurea，respectively．TheadditionofHepesexhibitednosignificanteffectsonneutrallipidsaccumulationinallofthethreenitrogenoussourcesafternitrogenstarvation．Keywords：ChloreUapyreno
