濟(jì)寧青山羊性早熟候選基因研究

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1、本研究由國(guó)家高技術(shù)研究發(fā)展計(jì)劃(863)(No．2006AAlOZl39)、國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專(zhuān)項(xiàng)資金(CARS-39)、國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(973)(No．2006CBl02105)、國(guó)家自然科學(xué)基金項(xiàng)目(No．30871773)、中國(guó)農(nóng)業(yè)科學(xué)院北京畜牧獸醫(yī)研究所基本科研業(yè)務(wù)費(fèi)專(zhuān)項(xiàng)(No．2010jc一9)和中國(guó)農(nóng)業(yè)科學(xué)院首批優(yōu)秀科技創(chuàng)新團(tuán)隊(duì)專(zhuān)項(xiàng)資助。ThisworkwassupportedbyNmionalHighTechnologyResearchandDevelopmentProgramofChina(No．2006AAl02139)，theearmarkedfundf

2、orChinaAgricultureResearchSystem(CARS-39)，NationalKeyBasicResearchandDevelopmentProgramofChina(No．2006CBl02105)，NationalNaturalScienceFoundationofChina(No．30871773)，theFundamentalScientificResearchFundsofChineseAcademyofAgriculturalSciences(No．201ojc-9、andChineseAcademyofAgriculturalSciencesofthefir

3、stteamofoutstandingscientificandtechnologicalinnovationinspecialfunding．符號(hào)說(shuō)明英文縮寫(xiě)英文全名中文全名G刪gonadotropin-releasinghormone促性腺激素釋放激素FSHFolliclestimulatinghormone促卵泡素LHLuteinizinghormone促黃體素bpbasepair堿基對(duì)cDNAcomplementarydeoxyribonucleicacid互補(bǔ)脫氧核糖核酸dDav天kDaKilodalton千道爾頓PCRpolymerasechainreaction聚合酶鏈?zhǔn)椒磻?yīng)GA

4、BA’,-aminobutyrate丫．氨基丁酸ubiquitinA-52residueribosomalproteinfusionUBA52泛素．52氨基酸融合蛋白product1GDHlglutamatedehydrogenase1谷氨酸脫氫酶1GAPDHGlyceraldehyde-3-phosphatedehydrogenase3．磷酸甘油醛脫氫酶CDSCodingsequence編碼序列IU乙PCRreversetranscription-polymerasechainreaction反轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)ArgArginine精氨酸GlyGlycine甘氨酸DEPCdiethypyr

5、ocarbonate焦炭酸二乙酯PBSPhosphateBufferedSaline磷酸緩沖液目錄中文摘要??????????????????????????????????．1ABSTRACT?????????????????．．：??????????????????????????3一、文獻(xiàn)綜述????????????????????????????????．51．1我國(guó)山羊業(yè)發(fā)展?fàn)顩r???????????????????????????51．2初情期?????????????????????????????????51．3性早熟??????????????????????????????

6、???．61．4UBA52基因研究進(jìn)展??????????????????????????．．71．4．1泛素研究概況????????????????????????????71．4．2UBA52研究概況???????????????????????????81．5谷氨酸脫氫酶1基因???????????????????????????81．6本研究的目的與意義???????????????????????????9二、性早熟候選基因UBA52的多態(tài)性研究???????????????????102．1實(shí)驗(yàn)材料???????????????????????????????．102．1．1實(shí)驗(yàn)動(dòng)

7、物與樣品采集????????????????????????．102．1．2克隆載體與菌株??????????????????????????．．102．1．3重要儀器與設(shè)備??????????????????????????．102．1．4主要酶和試劑???????????????????????????．102．1．5主要數(shù)據(jù)庫(kù)和生物軟件???????????????????????．112．