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1、2期湯紹虎等:外源NO對(duì)滲透脅迫下黃瓜種子萌發(fā)、幼苗生長(zhǎng)和生理特性的影響421外源NO對(duì)滲透脅迫下黃瓜種子萌發(fā)、幼苗生長(zhǎng)和生理特性的影響湯紹虎,周啟貴,孫敏,毛薇(西南大學(xué)生命科學(xué)學(xué)院,重慶400715)摘要:【目的】探究外源NO對(duì)滲透脅迫下黃瓜(CucumissativusL.)種子萌發(fā)、幼苗生長(zhǎng)和生理特性的作用。【方法】在25%PEG-6000脅迫下,研究0.1和0.5mmol·L-1SNP(硝普鈉)對(duì)黃瓜種子萌發(fā)、幼苗生長(zhǎng)、葉片氧化損傷和保護(hù)酶活性的影響?!窘Y(jié)果】0.1mmol·L-1SNP處理能顯著緩解25%PEG-6000的脅迫傷害,使黃瓜種子萌發(fā)率提高0.24倍,
2、發(fā)芽指數(shù)提高0.86倍,活力指數(shù)提高3.01倍;幼苗株高、根長(zhǎng)和干重分別提高1.15,2.17和1.16倍;葉片Pro含量提高50.99%,SOD,CAT,POD和APX活性分別提高42.39%,66.58%,79.03%和116.39%,MDA含量降低80.50%。與0.1mmol·L-1SNP處理相比,0.5mmol·L-1SNP處理促進(jìn)黃瓜種子萌發(fā)和幼苗生長(zhǎng),緩解葉片氧化損傷和提高保護(hù)酶活性的作用明顯或顯著降低?!窘Y(jié)論】0.1mmol·L-1SNP能顯著促進(jìn)滲透脅迫下黃瓜種子萌發(fā)和幼苗生長(zhǎng),明顯緩解葉片氧化損傷,顯著提高SOD等保護(hù)酶活性;0.5mmol·L-1SNP的
3、有效作用減弱,甚至抑制SOD活性。關(guān)鍵詞:黃瓜;種子萌發(fā);一氧化氮(NO);滲透脅迫;氧化損傷EffectsofExogenousNitricOxideonSeedGermination,SeedlingGrowthandPhysiologicalCharacteristicsofCucumberUnderOsmoticPressureTANGShao-hu,ZHOUQi-gui,SUNMin,MAOWei(CollegeofLifeScience,SouthwestUniversity,Chongqing400715)Abstract:【Objective】Theobjec
4、tiveofthisstudywastoinvestigatetheeffectsofexogenousnitricoxideonseedgermination,seedlinggrowth,andphysiologicalcharacteristicsinosmoticallystressedcucumber(CucumissativusL.).【Method】Weused25%PEG-6000toproducetheosmoticpressure,underwhichtheeffectsofsodiumnitroprusside(SNP),anexogenousnitri
5、coxidedonor,weremeasuredonseedgermination,seedlinggrowth,oxidativedamagetoleaves,andprotectiveenzymeactivitiesincucumber.WecarriedouttheaforementionedexperimentsattwoconcentrationsofSNP:0.1and0.5mmol·L-1.【Result】Thedamagetocucumbercausedbytheosmoticstressof25%PEG-6000wassignificantlyreduced
6、inthepresenceof0.1mmol·L-1ofSNP.Specifically,theseedgerminationrate,germinationindex,andvigorindexincreasedby0.24-fold,0.86-fold,and3.01-fold,respectively;plantheight,rootlength,anddryweightincreasedby1.15-fold,2.17-fold,and1.16-fold,respectively;prolinecontentandenzymeactivitiesofSOD,POD,C
7、AT,andAPXinleavesincreasedby50.99%,42.39%,66.58%,79.03%,and116.39%,respectively;thelevelofmalondialdehyde(MDA)inleavesdecreasedby80.50%.WhentheconcentrationofSNPwasincreasedto0.5mmol·L-1,weobservedadiminishingstimulativeeffectoncucumberseedgerminationand