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《波爾定對膠原誘導(dǎo)性關(guān)節(jié)炎大鼠的治療作用與對繼發(fā)性骨質(zhì)疏松骨微結(jié)構(gòu)的影響》由會員上傳分享,免費在線閱讀,更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、2.波爾定可以通過上調(diào)IL.10水平和下調(diào)IL。17的水平,抑制異常的自身免疫反應(yīng),改善關(guān)節(jié)滑膜炎性病變,這可能是其發(fā)揮作用的機制之一。關(guān)鍵詞:波爾定,類風(fēng)濕關(guān)節(jié)炎,膠原誘導(dǎo)性關(guān)節(jié)炎,骨質(zhì)疏松Ef扎ctsofBoldine1陌eatmentofOsteoporosisBoneMicrostructureinCollagenInducedArthritisRatsABSTRACTObjectiVe:Todetecte髓ctsofb01dineinthecollageninducedanhritis(CIA)ratmodelandthepossiblemechanis
2、mofaction.Methods:TheCIAratmodelwasestablishedinanrats,exceptthoseintheblankcontrolgroupusingboVinetypeIIcollagenaJldincompleteFreund、sa由uVant.ThentheratsweresubdiVidedintothef01lowinggroups:(1)blankcontrolgroup;(2)CIAmodel;(3)MTXgroup(3mg瓜g,twice/week);(4)thelowdoseofb01dinegroup(2mg
3、/l(∥d);(5)thehighdoseofboldinegroup(4mg/l(∥d).DmgtreatmentwasgiVenorallybegiIⅡ1ing15daysaRerthefirstimmunizationfor28days.Dumgtheperiodofthedrugtreamlent,thegeneralsituationofratswasobseⅣed,ratbodyweightmeasured,AIchangesjudgedandanHeswellingdegreemeasuredwiththeVemiercaliper.ARerthee
4、ndoftreatment,theexpression1eVelsoftheinflammato巧cytokinesinserumbyeI磁ymelinkedimmunosorbentmethodsuchasIL一1OwasperfomedinCIArats.5Meanwhile,HEstainingwasexecutedtoobserVethehistopath0109ychangesoftheanⅪeandimmunohistochemicalstainingmethodcarriedouttodetecttheexpI.essionleVelofIL一1Oa
5、ndIL一17inlocalratanl(1ejoint.Aswellasindicesofthymusandspleenwasobse]rVed.AllofthesewerecompletedtoeValuatetheefjfectofb01dineandexploretheirmechanism.Thentakethevertebrae(1umbar)andfemurforthedetectiononBMDandmicro—CT.Results:lEValuationan¨tisindeXandgeneralstate:Comparedwithblankcon
6、trolgroup,ratsofmodelgrouphadsignificantlyweightloss,actiVitiesreducedandspiritwaspoor.ProgressionofarthritissyIllptomaRerbuildingespeciallyasthesymmetrichind1imbsjoimsignificantly.Thepeakofanntisperf.oHIlanceappearsin4weeksaRertheimmune,thengraduallyintothechronicphasewithjointstifme
7、ssanddefomity.2Anl(1ejointswellingandhistopath0109icalresultsshowed:thehighdoseofboldinegroupcouldsigni6cantlyaUeViatethedegreeofinflammationinmodelrats.Duringtreatment,onday1O,AIValuesofhighdoseofboldinegroupwaslowerthanthatoftheCIAgroup(尸
8、feach