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1、安徽醫(yī)科大學(xué)碩士/博士學(xué)位論文分類號(hào)：密級(jí)：UDC：編號(hào)：學(xué)位論文褪黑素增強(qiáng)順鉑對(duì)人食管癌細(xì)胞的增殖抑制作用及其可能機(jī)制SynergisticallyAntiproliferativeEffectofMelatoninandCisplatinonHumanEsopHagealCancerCellLinesandthePossibleMechanism劉玲玲指導(dǎo)教師姓名孫國(guó)平教授安徽醫(yī)科大學(xué)（至院、所）申請(qǐng)學(xué)位級(jí)別碩士專業(yè)名稱腫瘤學(xué)提交論文日期2013-04論文答辯日期2013-05學(xué)位授予單位和日期安徽醫(yī)科大學(xué)答辯委員會(huì)主席評(píng)閱人2013年05月1安徽醫(yī)科大學(xué)碩士/博士學(xué)位論文
2、安徽醫(yī)科大學(xué)ANHUIMEDICALUNIVERSITY碩士學(xué)位論文論文題目褪黑素增強(qiáng)順鉑對(duì)人食管癌細(xì)胞的增殖抑制作用及其可能機(jī)制SynergisticallyAntiproliferativeEffectofMelatoninandCisplatinonHumanEsopHagealCancerCellLinesandthePossibleMechanism作者姓名劉玲玲指導(dǎo)老師孫國(guó)平教授學(xué)科專業(yè)腫瘤學(xué)研究方向腫瘤個(gè)體化治療論文工作時(shí)間2011年12月至2013年4月2安徽醫(yī)科大學(xué)碩士/博士學(xué)位論文學(xué)位論文獨(dú)創(chuàng)性聲明本人所呈交的論文是我個(gè)人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研
3、究成果。據(jù)我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過(guò)的研究成果。與我一同工作的同志對(duì)本研究所做的任何貢獻(xiàn)均已在論文中作了明確說(shuō)明并表示謝意。學(xué)位論文作者簽名：日期：學(xué)位論文使用授權(quán)聲明本人完全了解安徽醫(yī)科大學(xué)有關(guān)保留、使用學(xué)位論文的規(guī)定：學(xué)校有權(quán)保留學(xué)位論文并向國(guó)家主管部門或其指定機(jī)構(gòu)送交論文的電子版和紙質(zhì)版，有權(quán)允許論文進(jìn)入學(xué)校圖書館被查閱，有權(quán)將學(xué)位論文的內(nèi)容編入有關(guān)數(shù)據(jù)庫(kù)進(jìn)行檢索，有權(quán)將學(xué)位論文的標(biāo)題和摘要匯編出版。愿意將本人的學(xué)位論文提交《中國(guó)博士學(xué)位論文全文數(shù)據(jù)庫(kù)》、《中國(guó)優(yōu)秀碩士學(xué)位論文全文數(shù)據(jù)庫(kù)》和《中國(guó)學(xué)位論文全文數(shù)據(jù)庫(kù)》中全
4、文發(fā)表，并可以以電子、網(wǎng)絡(luò)及其他數(shù)字媒體形式公開出版，并同意編入CNKI《中國(guó)知識(shí)資源總庫(kù)》，在《中國(guó)博碩士學(xué)位論文評(píng)價(jià)數(shù)據(jù)庫(kù)》中使用和在互聯(lián)網(wǎng)上傳播。保密的學(xué)位論文在解密后適用本規(guī)定。學(xué)位論文作者簽名：導(dǎo)師簽名：日期：日期：3安徽醫(yī)科大學(xué)碩士/博士學(xué)位論文目錄中文摘要···················································7英文摘要···················································81.前言·············································
5、········102.實(shí)驗(yàn)材料·················································82.1細(xì)胞株·················································122.2藥物及試劑·············································122.3儀器與設(shè)備·············································133.實(shí)驗(yàn)方法················································143.1
6、細(xì)胞的培養(yǎng)與傳代·······································143.1.1細(xì)胞的凍存和復(fù)蘇·····································143.1.2細(xì)胞換液·············································153.1.3細(xì)胞傳代·············································163.2細(xì)胞活力的測(cè)定········································163.3體外增殖抑制實(shí)驗(yàn)················
7、······················163.4藥物相互作用指數(shù)評(píng)價(jià)藥物相互作用······················173.5集落克隆染色·········································173.6流式細(xì)胞術(shù)檢測(cè)細(xì)胞凋亡································183.7WesternBlot檢測(cè)Bcl-2、Bax蛋白表達(dá)····················123.7.1食管癌細(xì)胞Eca109總蛋白的提取········
