大鼠骨髓間充質(zhì)干細(xì)胞體外誘導(dǎo)分化為肝細(xì)胞

《大鼠骨髓間充質(zhì)干細(xì)胞體外誘導(dǎo)分化為肝細(xì)胞》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫。

1、瀘州醫(yī)學(xué)院學(xué)報2010年第33卷第l期JournalofLuzhouMedicalCoUegeVo1．33No．12010DIFFEIENTIATIONOFMARROWBONEMESENCHYMALSTEMCELLSINTOHEPATOCYTESFROMRATINVITROWangZhongqiong，LvMuhan，XiaShiping，ZhongXiaolin，LiChangpingDepartmentofGastroenterology，theAfiliatedHospitalofLuzhouMedicalColle

2、ge，Luzhou，646000，chinaAbstractObjectives：Tostudythepotentialofhepatocytegrowthfactor(HGF)，acidfibroblastgrowthfactorfaFGF)andoncostatinM(OSM)toinduceMSCsdifferentiateintohepatocytesandtheirroles．Methods：MSCsweretreatedwithHGF，aFGFandOSM．DetectingtheexpressionofAFP

3、，ALBandureawasperformedbyehe—mi—luminescenttechnique，RT-PCRmethodandglutamicaciddehydrogenasetechnique．Results：AFPexpressionemergedineverygroupexceptcontrolgrouprPl

4、OSMandaFGFcaninduceMSCsdifferentiateintohepatocyteswiththeexpressionofAFP，ALBandexcretingurea，whileaFGFandOSMcannotinduceMSCsdif-ferentiateintomaturehepatocytewithoutHGF．KeywordsBonemarrowmesenchymalstemcells；Hepatocytegrowthfactor；Acidicfibroblastgrowthfactors；On

5、costatinM：HepatocytesCLCNamberR734．2DocumentCodeAArticleID10O0—2669(2010)1-0040-05大鼠骨髓間充質(zhì)干細(xì)胞體外誘導(dǎo)分化為肝細(xì)胞王忠瓊，夏世萍，劉彥，鐘曉琳，李昌平(瀘州醫(yī)學(xué)院附屬醫(yī)院消化內(nèi)科，四川1瀘外I646000)摘要目的：研究HGF、aFGF及OSM誘導(dǎo)MSCs成為肝細(xì)胞的能力及其在分化中的作用。方法：用HGF、aFGF、OSM誘導(dǎo)分化MSCs后，用化學(xué)發(fā)光法、R．T—PCP．法及谷氨酸脫氫酶法檢測細(xì)胞AFP、ALB及尿素的表達(dá)情況。結(jié)果：

6、除對照組外其余各組細(xì)胞均有AFP表達(dá)(Pl

7、lrejectionwhichhasaeffectivetherapeuticmeasureforliverfailure，butthisgreatimpactonitsclinicalapplication【．Accord-therapyisfaeedwithhepaticsourceshortage，ingly，searchingfororiginalhep—atocellularsourceisimmunotoxicityandexpensivetreatment．Hepatocytedebatedintherese

8、archareahotly．Marrowbonemese-transplantation，(HCT)isoneofthemethodswhichnchymalstemcells(MSCs)candifferentiateintobone，replacethewholelivertransplantati