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《皺紋盤(pán)鮑內(nèi)臟酶的酶學(xué)性質(zhì)及褐藻膠裂解酶的分離純化》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫(kù)。
1、第31卷第1期水產(chǎn)學(xué)報(bào)Vol.31,No.12007年1月JOURNALOFFISHERIESOFCHINAJan.,2007文章編號(hào):1000-0615(2007)01-0015-08皺紋盤(pán)鮑內(nèi)臟酶的酶學(xué)性質(zhì)及褐藻膠裂解酶的分離純化康平,汪秋寬,宋琳琳,徐玲(大連水產(chǎn)學(xué)院食品工程系遼寧省水產(chǎn)品加工及綜合利用重點(diǎn)開(kāi)放實(shí)驗(yàn)室,遼寧大連116023)摘要:采用(NH4)2SO4分段鹽析、透析、陰離子(DEAE252)交換柱層析、SephadexG2200凝膠柱層析等分離純化技術(shù),通過(guò)SDS2PAGE電泳分析了皺紋盤(pán)鮑
2、內(nèi)臟酶的組成,結(jié)果表明鮑內(nèi)臟酶主要含有兩種褐藻膠裂解酶Ⅰ,Ⅱ,一種纖維素酶,一種瓊脂酶。對(duì)酶的酶學(xué)性質(zhì)分析結(jié)果表明兩種褐藻膠裂解酶Ⅰ,Ⅱ的最適pH分別為8.6,7.2,最適溫度為35℃,分子量分別為35.2ku,67ku;兩種褐藻膠裂解酶的熱穩(wěn)定性比較差,且易受金屬離子影響;纖維素酶的最適pH為5.0,最適溫度為40℃。并確定了皺紋盤(pán)鮑內(nèi)臟酶分離純化的方法及參數(shù),為進(jìn)一步研究鮑內(nèi)臟復(fù)合酶的性能提供了基礎(chǔ)參數(shù)。關(guān)鍵詞:皺紋盤(pán)鮑;褐藻膠裂解酶;纖維素酶;純化中圖分類(lèi)號(hào):Q814;S917文獻(xiàn)標(biāo)識(shí)碼:ACharacte
3、risticsofenzymesextractedfromabalone(HaliotisdiscushannaiIno)intestineandpurificationofthealginatelyaseKANGPing,WANGQiu2kuan,SONGLin2lin,XULing(KeyandOpenLaboratoryofAquaticProductProcessingandUtilizationofLiaoningProvince,DepartmentofFoodEngineering,DalianFi
4、sheriesUniversity,Dalian116023,China)Abstract:Theenzymesextractedfromabalone(HaliotisdiscushannaiIno)intestinewerestudiedforfindingthealginateandcellulosedegradationenzymes.Theenzymeswereseparatedandpartiallypurifiedfromabaloneintestinebyusingammoniumsulfatep
5、recipitations,andsuccessivefractionationonDEAE252,SephadexG2200columnchromatography.Theresultsshowedthattheirwerefourtypesofenzymeseparated,whichwerealginatelyaseⅠ,lyaseⅡ,cellulaseandagarase.ThecharacteristicsofalginatelyaseⅠ,lyaseⅡandcellulaserelatedwithpH,t
6、emperatureandionswerealsodeterminedandtheresultsindicatedthatalginatelyaseⅠhasthemaximumactivityatpH8.6and35℃.AlginatelyaseⅡhasmaximumactivityatpH7.2and35℃.CellulasehasmaximumactivityatpH5.0and40℃.ThealginatelyaseⅠandlyaseⅡwerenotstableathightemperature.Thecr
7、udeenzymesexstractedcouldbeobviously2+3+2+2++activatedbyMn,Al,Ca,CuandinhibitedbyNa.SDS2PAGEwasusedforestimatingthemolecularweightsofenzymesandthefindingsshowedthatthemolecularweightsofalginatelyaseⅠ,Ⅱweretobe35.2ku,67ku.Thefindingsindicatedthatthereweretwoty
8、pesofalginateenzymecouldbeobtainedfromabalone(HaliotisdiscushannaiIno)intestineandthestudiesestablishedthepurificationsteps收稿日期:2006206222資助項(xiàng)目:國(guó)家“九四八”項(xiàng)目(2005-Z23);遼寧省科技廳攻關(guān)項(xiàng)目(2004209001);大
