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《Springer.Advances.in.Biochemical.Engineering.vol.76.Biotechnology.Modern.Adva》由會員上傳分享，免費在線閱讀，更多相關內(nèi)容在學術論文-天天文庫。
1、PrefacePrefaceDuetotheirversatilityandresolution,chromatographicseparationsofcomplexmixturesofbiologicalsareusedformanypurposesinacademiaandindustry.Ifanything,recentdevelopmentsinthelifescienceshaveincreasedtheinterestandneedforchromatographybeitforqualitycontrol,proteomicsorthedown-streamproces
2、singofthehighvalueproductsofmodernbiotechnology.How-ever,themany“challenges”ofpresentdaychromatographyandespeciallyoftheHPLCofbiomacromoleculessuchasproteins,arealsopresentinthemindofanypractitioner.Infact,someoftheselatterweresuchhindrancesthatmuchresearchwasnecessaryinordertoovercomeandcircumve
3、ntthem.Thisbookintroducesthereadertosomeoftherecentlyproposedsolutions.Capillaryelec-trochromatography(CEC),forexample,thelatestandmostpromisingbranchofanalyticalchromatography,isstillhinderedfromfindingbroaderapplicationbydifficultiesrelatedtosomethingassimpleasthepackingofasuitablecolumn.Thelat
4、estsolutionsforthisbutalsothestateofartofCECingeneralaredis-cussedinthechapterwrittenbyFrantisekSvec.Thedifficultyofcombiningspeed,resolutionandcapacitywhenusingtheclassicalporousbeadtypesta-tionaryphaseshasevenbeencalledthe“dilemmaofproteinchromatography”.Muchprogresshasbeenmadeinthisareabythead
5、ventofmonolithicandrelat-edcontinuousstationaryphases.Thecomplexnatureofmanyofthesamplestobeanalyzedandseparatedinbiochromatographyoftenrequirestheuseofsomehighlyspecific(“affinity”)ligands.Sincetheycanberaisedinaspecificmannertomanybioproducts,proteinligandssuchasantibodieshaveallowedsomeverysel
6、ectivesolutionsinthepast.However,theyalsoareknowntohavesomedis-advantages,includingtheimmunogenicity(toxicity)ofligandscontaminatingthefinalproducts,orthelowstabilityofsuchligands,whichpreventsrepeatedusageoftheexpensivecolumns.Thischallengemaybeovercomeby“molecularimprinting”,atechniques,whichus
7、espurelychemicalmeanstocreatethe“affinity”interaction.Finallyweweremosthappytohavetwoauthorsfromindustryjoinustoreportontheirexperiencewithchromatographyasacontin-uouspreparativeprocess.Readersfromvariousfieldsthuswill
