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1、PrefacePrefaceDuetotheirversatilityandresolution,chromatographicseparationsofcomplexmixturesofbiologicalsareusedformanypurposesinacademiaandindustry.Ifanything,recentdevelopmentsinthelifescienceshaveincreasedtheinterestandneedforchromatographybeitforqualitycontrol,proteomicsorthedown-streamp
2、rocessingofthehighvalueproductsofmodernbiotechnology.How-ever,themany“challenges”ofpresentdaychromatographyandespeciallyoftheHPLCofbiomacromoleculessuchasproteins,arealsopresentinthemindofanypractitioner.Infact,someoftheselatterweresuchhindrancesthatmuchresearchwasnecessaryinordertoovercomea
3、ndcircumventthem.Thisbookintroducesthereadertosomeoftherecentlyproposedsolutions.Capillaryelec-trochromatography(CEC),forexample,thelatestandmostpromisingbranchofanalyticalchromatography,isstillhinderedfromfindingbroaderapplicationbydifficultiesrelatedtosomethingassimpleasthepackingofasuitab
4、lecolumn.ThelatestsolutionsforthisbutalsothestateofartofCECingeneralaredis-cussedinthechapterwrittenbyFrantisekSvec.Thedifficultyofcombiningspeed,resolutionandcapacitywhenusingtheclassicalporousbeadtypesta-tionaryphaseshasevenbeencalledthe“dilemmaofproteinchromatography”.Muchprogresshasbeenm
5、adeinthisareabytheadventofmonolithicandrelat-edcontinuousstationaryphases.Thecomplexnatureofmanyofthesamplestobeanalyzedandseparatedinbiochromatographyoftenrequirestheuseofsomehighlyspecific(“affinity”)ligands.Sincetheycanberaisedinaspecificmannertomanybioproducts,proteinligandssuchasantibod
6、ieshaveallowedsomeveryselectivesolutionsinthepast.However,theyalsoareknowntohavesomedis-advantages,includingtheimmunogenicity(toxicity)ofligandscontaminatingthefinalproducts,orthelowstabilityofsuchligands,whichpreventsrepeatedusageoftheexpensivecolumns.Thischallengemaybeovercomeby“moleculari
7、mprinting”,atechniques,whichusespurelychemicalmeanstocreatethe“affinity”interaction.Finallyweweremosthappytohavetwoauthorsfromindustryjoinustoreportontheirexperiencewithchromatographyasacontin-uouspreparativeprocess.Readersfromvariousfieldsthuswill
