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《Springer.Freitag.R.(ed.).Modern.Advances.in.Chromatography.(Springer.2002)(T)(251s)》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、PrefacePrefaceDuetotheirversatilityandresolution,chromatographicseparationsofcomplexmixturesofbiologicalsareusedformanypurposesinacademiaandindustry.Ifanything,recentdevelopmentsinthelifescienceshaveincreasedtheinterestandneedforchromatographybeitforqualitycontrol,proteomicsorthedown-strea
2、mprocessingofthehighvalueproductsofmodernbiotechnology.How-ever,themany“challenges”ofpresentdaychromatographyandespeciallyoftheHPLCofbiomacromoleculessuchasproteins,arealsopresentinthemindofanypractitioner.Infact,someoftheselatterweresuchhindrancesthatmuchresearchwasnecessaryinordertooverc
3、omeandcircumventthem.Thisbookintroducesthereadertosomeoftherecentlyproposedsolutions.Capillaryelec-trochromatography(CEC),forexample,thelatestandmostpromisingbranchofanalyticalchromatography,isstillhinderedfromfindingbroaderapplicationbydifficultiesrelatedtosomethingassimpleasthepackingofa
4、suitablecolumn.ThelatestsolutionsforthisbutalsothestateofartofCECingeneralaredis-cussedinthechapterwrittenbyFrantisekSvec.Thedifficultyofcombiningspeed,resolutionandcapacitywhenusingtheclassicalporousbeadtypesta-tionaryphaseshasevenbeencalledthe“dilemmaofproteinchromatography”.Muchprogress
5、hasbeenmadeinthisareabytheadventofmonolithicandrelat-edcontinuousstationaryphases.Thecomplexnatureofmanyofthesamplestobeanalyzedandseparatedinbiochromatographyoftenrequirestheuseofsomehighlyspecific(“affinity”)ligands.Sincetheycanberaisedinaspecificmannertomanybioproducts,proteinligandssuc
6、hasantibodieshaveallowedsomeveryselectivesolutionsinthepast.However,theyalsoareknowntohavesomedis-advantages,includingtheimmunogenicity(toxicity)ofligandscontaminatingthefinalproducts,orthelowstabilityofsuchligands,whichpreventsrepeatedusageoftheexpensivecolumns.Thischallengemaybeovercomeb
7、y“molecularimprinting”,atechniques,whichusespurelychemicalmeanstocreatethe“affinity”interaction.Finallyweweremosthappytohavetwoauthorsfromindustryjoinustoreportontheirexperiencewithchromatographyasacontin-uouspreparativeprocess.Readersfromvariousfieldsthuswill
