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1、分類號：Q617密級：無UDC：570學校代碼：11065碩士學位論文論文題目下丘腦室旁核orexin-A對大鼠體重和自由活動的影響及機制楊麗媛指導(dǎo)教師徐珞教授學科專業(yè)名稱病理生理學論文答辯日期2015年11月28日摘要目的：探討室旁核（PVN）微量注射orexin-A對大鼠體重的影響及機制。方法：采用PVN埋管、PVN微量注射，EchoMRI900體成分分析儀分析身體成分等方法，觀察室旁核微量注射orexin-A對大鼠體重、身體成分和自由活動的影響。結(jié)果：與注射人工腦脊液對照組相比，下丘腦PVN微量注射orexin-A，大鼠體重明顯減輕（P<0.05），但大鼠攝食量無明顯差異(P﹥0.05
2、)。實驗結(jié)束后6天，PVN微量注射orexin-A組大鼠體重仍顯著低于注射前（P<0.05），而人工腦脊液對照組大鼠體重比注射前顯著增加（P<0.05）；與注射人工腦脊液對照組大鼠比較，PVN微量注射Orexin-A，大鼠肌肉組織、脂肪組織和非脂肪組織均顯著降低（P<0.05），但注射orexin-A的大鼠降低更明顯。與注射人工腦脊液對照組相比，PVN微量注射orexin-A，大鼠的攝食轉(zhuǎn)化率（攝食量/體重）顯著降低（P<0.05）。下丘腦PVN微量注射orexin-A，大鼠水平運動量顯著增加（P<0.05）。結(jié)論：室旁核orexin-A可通過增加大鼠活動量而產(chǎn)生負能量平衡，體重減輕。碩士研
3、究生：楊麗媛（病理生理學）指導(dǎo)教師：徐珞教授關(guān)鍵詞：Orexin-A；PVN；非運動性活動產(chǎn)熱；負能量平衡；體重ABSTRACTObjective:Toinvestigatetheeffectoforexin-Amicroinjectedintotheparaventricularnucleusofthehypothalamus(PVN)onbodyweightinrats.Methods:ChronicguidecannulaewereimplantedintoPVNofrats.Orexin-Awasmicroinjectedintothebrainsite.Theeffectofore
4、xin-Aonbodyweightinratswasdetected.Results:Thelossofbodyweightinorexin-A-treatedratswasmoresignificantlythantheirvehicle-injectedcounterparts（P<0.05）,buttheyhadnodifferenceinfoodintake(P﹥0.05).Theratsweremoresignificantlyactiveafterintra-PVNorexinmicroinjectioncomparedtovehicle（P<0.05）.Theseresults
5、supporttheconceptthatorexinergicagentshavethepotentialtoproducenegativeenergybalancethroughincreasingphysicalactivity.Conclusion:Orexin-Amicroinjectedintotheparaventricularnucleusofthehypothalamus(PVN)caninduceweightlossinratsthroughproducingnegativeenergybalanceviatheincreaseofphysicalactivity.Pos
6、tgraduatestudent:Li-YuanYang(Pathophysiology)Directedby:Prof.LuoXuKeywords：Orexin-A;PVN;Non-exerciseactivitythermogenesis;Negativeenergybalance;Bodyweight目錄引言······································································································1第1章材料與方法······························
7、····················································31.1主要實驗材料·····················································································31.2主要實驗方法·················································
