differentially activation of tlr3 by very virulent and attenuateg virulent infectious bursal disease virus in the bursa of fabricuis in commercial chickens論文

《differentially activation of tlr3 by very virulent and attenuateg virulent infectious bursal disease virus in the bursa of fabricuis in commercial chickens論文》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。

1、學(xué)校代碼10608學(xué)號(hào)L201207101001分類號(hào)S855.3密級(jí)公幵GuangxiUniversityforNationalities碩士學(xué)位辦丈DifTerentiallyactivationofTLR3byveryvirulentandattenuatedvirulentInfectiousBursalDiseasevirusinthebursaofFabricuisincommercialchickens研究生姓名：康思那導(dǎo)師姓名職稱：何秀苗教授學(xué)科專業(yè)：生物化學(xué)與分子生物學(xué)所屬學(xué)院：海洋與生物技術(shù)學(xué)院年級(jí)：2

2、012級(jí)論文完成時(shí)間：201S年6月?學(xué)??S855.3????????ウ???????仈??DifferentiallyactivationofTLR3byveryvirulentandattenuatedvirulentInfectiousBursalDiseasevirusinthebursaofFabricuisincommercialchickensуъ?????о??????ウ???????????ウ??????????????????→??2013?4??2015?5?Differentiallyactiva

3、tionofTLR3byveryvirulentandattenuatedvirulentInfectiousBursalDiseasevirusinthebursaofFabricuisincommercialchickensAbstractToll-likeReceptor3(TLR3)isoneoftheTLRswhoseligandisdoublestrandRNA(dsRNA).Infectiousbursaldiseasevirus(IBDV),whichisadsRNAvirus,couldberecogni

4、zedbyTLR3.InfectiousBursalDisease(IBD)causessignificantlossestothepoultryindustries,suchashighmortality,andimmunosuppressioninyoungchickens.Uptonow,littleisknownonthesignalingpathway,mechanisms,anddeterminesthefunctionofchTLR3onIBDVinfectioninvivo.Inthisstudy,itis

5、importantandessentialtostudytherelationshipofChTLR3signalingpathwaywithIBDV-infectionincommercialchickens.Aspreviously,ourgroupwassuccessfullycloningchicken?stolllike-receptor3gene(GX-sh-chTLR3).Inthisstudy,thepolyclonalantibodyagainstChTLR3waspreparedbyimmunizati

6、onofBALB/cmicewithGX-sh-chTLR3protein,whichwasexpressedinE-coli.AnIndirectEnzymeLink-immunosorbentAssay(ELISA)wasdevelopedfordetectionoftheantibodylevelinmiceserum.Theresultsshowedthatamongvariousserumdilution,1:800immuneserumdilutionwith1:400antigen(chTLR3-LRRrec

7、ombinantprotein)dilutionarethebestconditionforELISAdetection,whereas1:100immuneserumdilutionwith1:400immuneserumdilutionshowedthelowestpositive(2.21ratio).ThisresultsuggeststhatthepolyclonalantibodypreparedinthisstudyhasahighsensitivityfordetectingofrecombinantchT

8、LR3-LRRbyELISA.TherelationshipofchTLR3andIBDVinfectionwascarriedoutinnon-vaccinated4-week-oldcommercialchickensbychallengingwithvvIBDVstrainNN1172andatt