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《橄欖綠鏈霉菌E86木聚糖酶的固定化及低聚木糖制備研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、中國農(nóng)業(yè)大學(xué)博士學(xué)位論文橄欖綠鏈霉菌E-86木聚糖酶的固定化及低聚木糖制備研究姓名：艾志錄申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：食品科學(xué)指導(dǎo)教師：李里特;江正強(qiáng)20040601中國農(nóng)業(yè)大學(xué)博士學(xué)位論文摘要酶工藝簡(jiǎn)單、條件溫和、通過改變環(huán)境pH值可以很容易的實(shí)現(xiàn)在溶解狀態(tài)下進(jìn)行酶反應(yīng)，降低固定化酶擴(kuò)散限制，改善酶與底物的親合性，而在不溶狀態(tài)下進(jìn)行酶與酶解產(chǎn)物的分離和重復(fù)利用。采用稀堿液預(yù)處理玉米芯則可以簡(jiǎn)單有效地脫除木聚糖結(jié)合的木質(zhì)素。改善底物與酶的親和性，消除了木質(zhì)素的屏蔽效應(yīng)對(duì)固定化酶作用的影響，兩者的結(jié)合使得固定化酶能高效利用玉米芯中的木聚糖生產(chǎn)低聚木糖。本研究為利用固定化酶直接水解玉米芯生產(chǎn)低聚
2、木糖開辟了一種全新的思路，為工業(yè)化利用固定化酶法生產(chǎn)低聚木糖提供了基礎(chǔ)。因此可以期待其在工業(yè)化低聚木糖生產(chǎn)中發(fā)揮其應(yīng)有的作用。②在國際上首次報(bào)道采用環(huán)氧基載體固定化木聚糖酶得到良好效果．并經(jīng)連續(xù)水解玉米芯汽爆液生產(chǎn)低聚木糖證明了其可行性。關(guān)鍵詞：木聚糖酶固定化酶橄欖綠鏈霉菌聃6低聚本糖2AbstractAccordingtotherequestofpracticalapplications，thearticlestudfedtheimmobilizationtechniquewhichaimedtoovercomethekeytechnicalproblemsdudngtheindust
3、rialproductionofxylooligosacchafidesusingimmobilizedxylanase．Thebestcarrierswereselectedfromthirtykindsofcarriers．Theconditionsforimmobilizationwereoptimized．Theimmobilizedenzymesweresystematicallycharacterizedintermsoftheircatalyticactivity,kineticparameters，thermalstabilityandapplication，etc．Th
4、exylooligosaccharideswereproducedfromthehydrolysisofbothdilute—alkali—treatedcorncobandsteam-explodedcomeobliquorwithimmobilizedenzymes．Theoptimalhydrolysisconditionsforbothsubstrateswereinvestigated．Resultsshowthat：1．Amongthethirtykindscarriersstudied，fourcarriersshowedrelativelygoodimmobilizati
5、onseffectonthexylanasefromStreptomycesolivaceoviridisE-86,namelyEudragitS-100、EupergitCandmacroporouspolystyreneresinD380．2．Thisisthefirstreportonthemethodofimmobilizingenzymeusingreversibilitysoluble—insolublepolymerin0urcountry．ThepolymershowedagoodimmobilizingeffectonthexylanasefromStreptomyce
6、solivaceoviridisE-86．Theimmobilizedxylanaseretained91．84％ofitsactivityuponimmobilizationby1％EudragitS一100．Theactivityoftheimmobilizedenzymereachedto74．68U／ml，whichwashighestinourcountry．3．Thisisalsothefirstreportonxy]anaseimmobilizationusingoxirane-groupcarrier．When232Uoffreeexylanasewasimmobiliz
7、edusingof1gcarrierinthepresenceofpotassiumphosphatebuffer(1M，pH5．8)for36～48hours，therecoveryandimmobilizedxylanaseactivitywere35％and82U／g，respectively4．Comparedtothefreeenzyme，theoptimumpHofimmobilizedxylanasefromtheSt
