妊高征胎盤絨毛組織內皮素1及其受體的mRNA表達

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1、碩士學位論文摘要妊高征胎盤絨毛組織內皮素一1及其受體的mRNA表達摘要目的：檢測妊高征患者及正常妊娠產婦胎盤絨毛組織內皮素一1(ET一1)及其受體(ETA、ETB)的mRNA表達，探討其與妊高征的關系。方法：60例妊高征患者(妊高征組)和30例正常妊娠孕婦(正常妊娠組)，在剖宮產術中取胎盤絨毛組織，采用逆轉錄聚合酶鏈反應(RT—PCR)檢測絨毛組織ET—l、ETA和ETB的mRNA表達，并設立GAPDH內對照對PCR產物作半定量分析。結果：胎盤絨毛組織存在ET一1、ETA及ETBmRNA的表達，且妊高征組ET—l和ETAmRNA表達均明顯高于正常對照組，兩組間差

2、異有顯著性(P均

3、ETB作用的不同，ETA、ETB平衡的失調亦可能參與妊高征的發(fā)病。關鍵詞妊娠并發(fā)癥，高血壓，胎盤，ET一1，ETA，ETB，RNA，信使碩士學位論文摘要Expressionofendothelin-IandendothelinreceptorsmRNAinplacentalvillousfrompregnancyinducedhypertensionABSTRACTobjective：TodetectthemRNAexpressionofendothelin-I(ET-1)，ET-lreceptorsE1’AandETBinplacentavillousfrom

4、normalpregnancy(ro)andpregnancyinducedhypertension(PIH)．Andtoinvestigatetheroleofendothelin·1andendothelinreceptorsinthepathogenesisofpregnancyinducedhypertension．Methods：ThemRNAlevelsofET-1，ETAandETBweredeterminedusingRT-PCRandexpressedasarbitraryunitsaftercorrectionforcontrolGAPDHg

5、enemRNAlevelsinplacentavillousfrom30NPand60Pm．Results：ThemRNAexpressionofET-1，ETAandETBwerefoundinplacentafrombethPIHandNPThemRNAlevelsofET-1andETAweresignificantlyhigherinPIHthanthatinNP(p

6、TBwerefoundinplacenta．Theplacentaisshortofnerveregulationitself,SOweconsiderETasaparacrinefactorplaysallimportantroleinfetal-placentalcirculation．ET--ETRsystemmayparticipateinthenormaldevelopmentofplacentaandtheregulationofthefetal—placentMbloodvessels．2．肌lenET一1iscombinedwithETA．itc

7、anarose-intensevesselcontraction。BecausetheexpressionofET-IandETAmRNAwerebothsignificantlyhigherinplacentaofPIH，theresistanceoffetal．placentalcirculationwasincreased．and也eplacentawasshortofbloodandoxygen，andtheplacentamayreleaseaseriesoftoxicityfactors．Wbconsiderthismaybeoneofthereas

8、onsthatcause