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1、中國水稻科學(xué)(ChinJRiceSci),2013,27(3):223-230http://www.ricesci.cnDOI:10.3969/j.issn.1001-7216.2013.03.001223抑制OsAGO1a基因的表達(dá)導(dǎo)致水稻葉片近軸面卷曲李磊1,#薛薌1,#左示敏1陳宗祥1張亞芳1李前前1朱俊凱2馬玉銀3潘學(xué)彪1,*潘存紅2,*(1揚(yáng)州大學(xué)農(nóng)學(xué)院,江蘇揚(yáng)州225009;2江蘇里下河地區(qū)農(nóng)業(yè)科學(xué)研究所,江蘇揚(yáng)州225007;3揚(yáng)州職業(yè)大學(xué)生化工程系,江蘇揚(yáng)州225009;#共同第一作者;*通訊聯(lián)系人,E-mail
2、:shuidao@yzu.edu.cn;pancunhong@163.com)SuppressedExpressionofOsAGO1aLeadstoAdaxialLeafRollinginRiceLILei1,#,XUEXiang1,#,ZUOShi-min1,CHENZong-xiang1,ZHANGYa-fang1,LIQian-qian1,ZHUJun-kai2,MAYu-yin3,PANXue-biao1,*,PANCun-hong2,*(1AgriculturalCollege,YangzhouUniversity,
3、Yangzhou225009,China;2LixiaheAgriculturalResearchInstituteofJiangsuProvince,Yangzhou225007,China;3DepartmentofBiochemicalEngineering,YangzhouPolytechnicCollege,Yangzhou225009,China;#Theseauthorscontributeequallytothispaper;*Correspondingauthors,E-mail:shuidao@yzu.edu
4、.cn;pancunhong@163.com)LILei,XUEXiang,ZUOShimin,etal.SuppressedexpressionofOsAGO1aleadstoadaxialleafrollinginrice.ChinJRiceSci,2013,27(3):223-230.Abstract:Argonaute(AGO)proteinisthecoreeffectorsinRNA-inducedsilencingcomplexes(RISCs),playinganndththimportantroleinplan
5、tdevelopment.The2andthe11-12exonfragmentsofOsAGO1awereamplifiedindependentlytomaketwoRNAiconstructs:OsAGO1aI-1EandOsAGO1aI-2E.ToclarifythefunctionofOsAGO1ainricedevelopment,theexpressionofOsAGO1ahasbeensuppressedbyintroducingRNAiconstructstoNipponbarethroughAgrobacte
6、rium-mediatedtransformation.TheresultshowedthatthereducedexpressionofOsAGO1aleadtoadaxialleafrollingwithoutaffectingmainagronomictraits.Keywords:rice;OsAGO1a;polaritydevelopment;rolledleaf;agronomictraits李磊,薛薌,左示敏,等.抑制OsAGO1a基因的表達(dá)導(dǎo)致水稻葉片近軸面卷曲.中國水稻科學(xué),2013,27(3):223-230
7、.摘要:AGO蛋白是RNA誘導(dǎo)沉默復(fù)合體的核心元件,在植物生長發(fā)育過程中發(fā)揮著重要作用。通過分別特異性地擴(kuò)增OsAGO1a基因中第2外顯子和第11~12外顯子的片段,構(gòu)建了兩個干涉載體OsAGO1aI-1E和OsAGO1aI-2E。利用農(nóng)桿菌介導(dǎo)法轉(zhuǎn)化水稻品種日本晴,抑制OsAGO1a基因的表達(dá),借此研究OsAGO1a的功能及其對水稻生長發(fā)育的影響。結(jié)果表明,抑制OsAGO1a的表達(dá)導(dǎo)致葉片的近軸面卷曲而對其他主要農(nóng)藝性狀的影響不大。關(guān)鍵詞:水稻;OsAGO1a;極性發(fā)育;卷葉;農(nóng)藝性狀中圖分類號:Q786;S511.032文獻(xiàn)
8、標(biāo)識碼:A文章編號:1001-7216(2013)03-0223-08AGO(Argonaute)蛋白是RISC(RNA-inducedsiRNA,tasiRNA);AGO4結(jié)合重復(fù)相關(guān)siRNAsilencingcomplex,RNA誘導(dǎo)沉默復(fù)合體)的標(biāo)志(r
