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1、中國農(nóng)業(yè)科學(xué)2004,37<4):477-483ScientiaAgriculturaSinica甘藍(lán)型油菜SSR,ISSR標(biāo)記的遺傳多樣性及其與雜種表現(xiàn)的關(guān)系沈金雄’，’，傅廷棟’，楊光圣’(‘中國農(nóng)業(yè)科學(xué)院油料作物研究所，武漢430062;1華中農(nóng)業(yè)大學(xué)作物遺傳改良國家重點實驗室，武漢430070)摘要:利用SSR,ISSR標(biāo)記技術(shù)分析了3個甘藍(lán)型油菜自交不親和系及22個不同來源的父本品種的遺傳多樣性，及其與這些親本按NCII法配制雜種的產(chǎn)量性狀表現(xiàn)及雜種優(yōu)勢的關(guān)系。結(jié)果表明，墓于遺傳距離的UPGMA(非加權(quán)類平均法)聚類法可將25個親本材料分為6組
2、，SI-1300,SI-1320各為一組，來源于中國的2個父本品種為一組，SI-1310與來源于國外的其它品種分屬另3組。這種分組結(jié)果與材料的來源及系譜關(guān)系墓本一致。各組間親本在產(chǎn)量性狀、種子品質(zhì)性狀及物候期性狀上存在明顯差別。SSR,ISSR標(biāo)記的遺傳距離與雜種單株產(chǎn)量極顯著乓相關(guān)，但決定系數(shù)較小。SSR及ISSR標(biāo)記遺傳距離難以預(yù)測甘藍(lán)型油菜雜種產(chǎn)量及其優(yōu)勢.關(guān)鍵詞:甘藍(lán)型油菜;遺傳多樣性;雜種表現(xiàn);SSR;ISSRRelationshipBetweenHybridPerformanceandGeneticDiversityBasedonSSRand
3、ISSRinBrassicanapusL.SHENJin-xiong',2,FUTing-dongz,Guang-sheng2('OilCropsResearchInstitute,ChineseAcademyofAgriculturalSciences,Wuhan430062;'NationalKeyLaboratoryofCropGeneticImprovement,HuazhongAgriculturalUniversity,Wuhan430070)Abstract:Toinvestigatetherelationshipbetweengeneti
4、cdistance(GD)andhybridperformance,SSR(microsatellite)andISSR(intro-simplesequencerepeat)wereemployedtodetectthegeneticdiversityof3doublelowself-incompatiblelinesand22maleparentvarietiesinBrassicanapusfromdifferentorigins.HybridswereproducedinaNCIImatingdesignbyhand-pollination.Re
5、sultsindicatedthat25parentvarieties(lines)couldbedividedintosixgroupsbyUPGMA(un-weightedpairgroupmathematicsaverage)clusteringbasedonGD.SI-1300andSI-1320couldbesinglyclusteredintoonegroup,respectively.MalevarietiesfromChinacouldbedividedintoanothergroup.SI-1310andmalevarietiesfro
6、mforeigncountriescouldbedividedintootherthreegroups.Thegroupingwasgenerallyconsistentwithparentalpedigreesandgeographicalorigins.Significantdifferencesinyield,qualityandphenologicalperiodtraitswereobservedamongtheseparentgroups.Althoughhybridyieldperplantshowedsignificantpositive
7、correlationwithgeneticdistancebasedonSSRandISSRmarkers,butthedeterminationcoefficients(R2)werelow.ItappearedtobeunsuitableforusingthegeneticdistancebasedonSSRandISSRmarkerstopredictheterosisandhybridperformanceinBrassicanapus.Keywords:BrassicanapusL;Geneticdiversity;Hybridperform
8、ance;SSR;ISSR油菜是我國乃至全世界廣泛種植的油料作物。106ha，總
