Targeted genome modification of crop plants using a CRISPR-Cas system英文文獻(xiàn)資料

Targeted genome modification of crop plants using a CRISPR-Cas system英文文獻(xiàn)資料

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時(shí)間:2019-06-27

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1、CoRRESPoNDENCErevisionprocessofthiswork,anindependentCOMPETINGFINANCIALINTERESTSfrom14.5%to20.0%,asestimatedbybandstudyreportedthesimultaneousgenerationTheauthorsdeclarenocompetingfinancialinterests.intensities(Fig.1candSupplementaryofmultiplemutationsinmice7.Ourwork,1,31–311Meth

2、ods).CloningandsequencingoftheseWeiLi,FeiTeng,TiandaLi&QiZhoutogetherwiththemicework,demonstratesuncutbandsrevealedindelsinthetargeted1StateKeyLaboratoryofReproductiveBiology,thatitshouldbefeasibletoproducegene-OsPDSgene.ThehighestfrequencyofInstituteofZoology,ChineseAcademyoftar

3、getedmodelsinrodentsandprobablymutationswasobtainedwithansgRNASciences,Beijing,China.2UniversityofChineseothermammalianspeciesusingtheCRISPR-3with20nucleotides(nts)ofsequenceAcademyofSciences,Beijing,China.TheseCassystems.complementarytotheOsPDS-SP1targetauthorscontributedequally

4、tothiswork.e-mail:qzhou@ioz.ac.cnsite(P=0.039)(SupplementaryFig.1c,d).Note:SupplementaryinformationisavailableintheWetargetedanotherthreericegenesonlineversionofthepaper(doi/10.1038/nbt.2652).1.Jinek,M.etal.Science337,816–821(2012).(OsBADH2,Os02g23823andOsMPK2)and2.Cho,S.W.,Kim,S

5、.,Kim,J.M.&Kim,J.S.Nat.onewheatgene(TaMLO)(SupplementaryACKNOWLEDGMENTSBiotechnol.31,230–232(2013).3.Cong,L.etal.Science339,819–823(2013).Tables1and4)inprotoplasts,withindelThisstudywassupportedbygrantsfromtheNational4.Mali,P.etal.Science339,823–826(2013).frequenciesof26.5–38.0%(

6、SupplementaryBasicResearchProgramofChina2012CBA01300,5.Hwang,W.Y.etal.Nat.Biotechnol.31,227–229Fig.2andSupplementaryTable2).“StrategicPriorityResearchProgram”oftheChinese(2013).AcademyofSciencesXDA01020101,theNational6.Jiang,W.,Bikard,D.,Cox,D.,Zhang,F.&Marraffini,Thefrequencyofm

7、utationsinducedbyScienceFoundationofChina90919060andtheL.A.Nat.Biotechnol.31,233–239(2013).sgRNA:Cas9inOs02g23823waslowerMinistryofScienceandTechnologyofChina7.Wang,H.etal.Cell153,910–918(2013).(26.0%)thanthatinducedbyTALENs2011CBA01101(alltoQ.Z.).8.Geurts,A.M.etal.Science325,433

8、(2009).9.Tong,C.,Li,P.,Wu,N.L.,Yan,Y.&Yi

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