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《QuilA為佐劑的H1,H3亞型流感病毒多表位DNA疫苗免疫評(píng)價(jià)》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫。
1、QuilA為佐劑的H1、H3亞型流感病毒多表位DNA疫苗免疫評(píng)價(jià)靖杰1,2,魯會(huì)軍2*,劉昊1,2，劉存霞1,2，劉燕瑜1,2，任靜強(qiáng)1,2，陸飛2,3，金寧一1，2*【基金項(xiàng)目】863項(xiàng)目(No.2012AA02A403);973項(xiàng)目(No.2012CB722501);軍隊(duì)重點(diǎn)項(xiàng)目(No.BWS11J048)。*【通訊作者】金寧一，E-mail：ningyij@yahoo.com;魯會(huì)軍，E-mail：nakata0007@sina.com【作者簡介】靖杰(1986-)，男，碩士。主要研究方向：
2、動(dòng)物病毒學(xué)。(1,吉林大學(xué)畜牧獸醫(yī)學(xué)院吉林長春130062；2,軍事醫(yī)學(xué)科學(xué)院軍事獸醫(yī)研究所吉林長春130122；3吉林農(nóng)業(yè)大學(xué)吉林長春130118)【摘要】目的選用QuilA為佐劑免疫小鼠評(píng)價(jià)H1、H3亞型流感病毒多表位DNA疫苗的免疫效果。方法將pVAX1-H3-EHA-H1HA1new，pVAX1-H3-H1HAnew，pVAX1-EHA等三種候選核酸疫苗免疫Balb/C小鼠，分別對(duì)各免疫組小鼠進(jìn)行細(xì)胞免疫和體液免疫水平檢測。結(jié)果體液免疫結(jié)果表明，免疫后35天pVAX1-H3HA-EHA-H
3、1HA1new免疫組小鼠產(chǎn)生H1亞型流感特異性體液免疫應(yīng)答,且抗體水平略高于其它免疫組。細(xì)胞免疫結(jié)果表明，當(dāng)受到H1亞型流感病毒抗原刺激時(shí),免疫組均表現(xiàn)出特異性T淋巴細(xì)胞增殖反應(yīng)，各免疫組小鼠血清IL-2、IL-4、IL-10細(xì)胞因子水平明顯高于對(duì)照組（P<0.05）。結(jié)論H1、H3亞型流感多表位疫苗可誘導(dǎo)免疫小鼠產(chǎn)生特異性的體液和細(xì)胞免疫應(yīng)答?！娟P(guān)鍵詞】流感病毒；QuilA；DNA疫苗；免疫Immunologicalevaluationofmulti-epitopeDNAVaccineofinf
4、luenzavirussubtypeH1andH3withtheadjuvantQuilAJINGJie1,2,LUHui-jun2*,LIUHao1,2,LIUCun-xia1,2,LIUYan-yu1,2,RENJing-qiang1,2,LUFei2,3,JINNing-yi1,2*(1.CollegeofAnimalScienceandVeterinaryMedicine,JilinUniversity,Changchun130062,China;2.InstituteofMilitary
5、Veterinary,AcademyofMilitaryMedicalSciences,Changchun130122,China;3.CollegeofAnimalScience,JilinAgriculturalUniversity,Changchun130118,China)【Abstract】ObjectiveMicewereemployedtoevaluatetheimmunogenicityoftheH1/H3combinedmulti-epitopevaccinewiththeadj
6、uvantQuilA.MethodsTheBalb/CmicewereimmunedwithDNAvaccinepVAX1-H3-EHA-H1HA1new,pVAX1-H3-H1HAnewandpVAX1-EHArespectively.Andthenthehumoralimmunityandcellularimmunitylevelweredetected.ResultsTheresultsofhumoralimmunityshowedthatthemiceingrouppVAX1-H3HA-E
7、HA-H1HA1newshowedhigherELISAantibodylevelthanthemiceinotherimmunedgroups35dayspostinoculation.TheresultsofcellularimmunityshowedthatthemiceinepitopegroupinducespecificSIoftheepitope-relatedsubtypessignificantly,andtheDNAVaccineimmunedmiceshowedhighlev
8、elofthecytokineofIL-2,IL-4andIL-10.ConclusionTheresultsofimmunityexperimentsconfirmedthatcombinedmulti-epitopevaccinehadsignificantadvantagesoninductionofbothhumoralimmunityandcellularimmunity.【Keywords】Influenzavirus;QuilA;DNAvaccine;immunity
