資源描述：
《Chloroethene degradation and expression of Dehalococcoides dehalogenase genes in cultures originating from Yangtze sediments 》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、EnvironSciPollutRes(2015)22:3138–3148DOI10.1007/s11356-014-3574-4RESEARCHARTICLEChloroethenedegradationandexpressionofDehalococcoidesdehalogenasegenesinculturesoriginatingfromYangtzesedimentsIreneKranzioch&SelinaGanz&AndreasTiehmReceived:10June2014/Accepted:8September2014/Publishedonline:20Septembe
2、r2014#Springer-VerlagBerlinHeidelberg2014AbstractTheanaerobicDehalococcoidesspp.istheonlyIntroductionmicroorganismknowntocompletelydechlorinatethehazard-ouscompoundstetrachloroethene(PCE)ortrichloroetheneTheextensiveuseofchlorinatedethenesassolventsand(TCE)viadichloroethene(DCE)andvinylchloride(VC)
3、tosyntheticfeedstockshasresultedinwidespreadenvironmen-theterminalproduct,ethene.Inthisstudy,growthoftalcontamination.Theirstrongtoxicity,persistenceintheDehalococcoidesspp.(DHC)andtheexpressionofDHCenvironment,andcarcinogenicitymayposeseriousenviron-dehalogenasegenesweredemonstratedforYangtzeenric
4、h-mentalproblems.Groundwaterinthevicinityofindustrialmentcultures.Reductivedechlorinationofchloroethenesoc-sitesproducingorusingchlorinatedorganiccompoundsiscurredinYangtzesedimentwithouttheadditionofanyexter-oftencontaminatedwithchlorinatedethenes(Akta?etal.nalauxiliarysubstrates.AllYangtzeenrichm
5、entcultures2012),andchlorinatedethenecontaminationhasbeenreport-completelydechlorinatedPCEandcis-DCEtoethene.Toedinsoilandgroundwaterworldwide,includinginGermany,investigateexpressionofthedehalogenasegenespceA,tceA,theUSA,Japan,andSouthKorea(SchmidtandTiehm2008;vcrA,andbvcA,aprotocolformessengerRNA
6、(mRNA)Carreón-Diazcontietal.2009;Iseetal.2011;Leeetal.2011).extractionfollowedbyreversetranscriptionandquantitativeInChina,theoccurrenceofchlorinatedethenesPCRanalysiswasestablished.Duringdechlorination,anin-(tetrachloroethene(PCE),trichloroethene(TCE),andvinylcreaseingenecopynumbersofpceA,tceA,and
7、vcrAwaschloride(VC))hasbeenreportedinsurfacewater(Lietal.observed.However,temporaryformationofmRNAwasonly2006;Hanetal.2011;Wolfetal.2013).InthelastfewmeasuredinthecaseofthedehalogenasegenestceAandvcrA.decad
