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1、萬方數(shù)據(jù)24JournalofAnimalScienceandVeterinaryMedicineV01.32No.22013枯草芽孢桿菌的分離篩選白延琴1,辛小玲2,來有志3,張效川4,張剛娟5,劉建峰6,辛亞平7(1.子長縣畜牧獸醫(yī)局史家畔畜牧獸醫(yī)站,陜西子長717300;2.富平縣畜牧獸醫(yī)工作站;3.陜西正大有限公司;4.吳起縣畜牧局;5.商南縣畜牧中心趙川獸醫(yī)站;6.陜西洛南縣四皓鎮(zhèn)獸醫(yī)站;7.西北農(nóng)林科技大學(xué)動(dòng)物科技學(xué)院)摘要:本文基于從秸稈垛底層土壤中分離枯草芽孢桿茵的目的,為發(fā)酵秸稈提供菌株,從陜西楊凌農(nóng)村秸稈垛中采取底層土樣并進(jìn)行分離。分離出11株茵,經(jīng)過形
2、態(tài)特征、生理生化特征等方法從中分離初步鑒定了3株疑似茵株。經(jīng)對(duì)液態(tài)發(fā)酵條件的優(yōu)化,對(duì)3株疑似茵進(jìn)行液態(tài)發(fā)酵試驗(yàn),以粗蛋白和粗纖維含量為標(biāo)準(zhǔn),篩選出一株發(fā)酵效果最好的菌株b一1進(jìn)行了16SrDNA序列分析,最終鑒定b一1菌株為枯草芽孢桿茵(Bacillussubtilis),其同源性≥99%。關(guān)鍵詞:枯草芽孢桿菌;篩選;鑒定;[中圖分類號(hào)]$816.6[文獻(xiàn)標(biāo)識(shí)碼]A[文章編號(hào)]1004—6704(2013)02-0024-04IsolationandScreeningofBacillusSubtilisBAIYan-qinl,XINXiao—lin92,LAIYOU—zhi
3、3,ZHANGXiao-chuan4,ZHANGGang-juan5,LIUJian—fen96,XINYa—pin97(1.ShijiapanStationofZichangHusbandryandVeterinaryBureau,ZichangShaanxi717300,China;2.FupingCountyAnimalHusbandryandVeterinaryStation;3.ShaanxiChiaTaiCo.,Ltd.;4.WuqiCountyAnimalHusbandryBureau;5.ZhaochuanVeterinaryStationofAnimalS
4、cienceCenterinShangnanCounty;6.SihaoVeterinaryStationinLuonanCounty:7.CollegeofAnimalScienceandTechnology。NorthwestA&F[Iniversity)Abstract:ThisstudyaimedtoisolateBacillussubtilisfromthebottomsoilofwheatstrawandprovidestrainsforstrawfer-mentation,thesoilsamplewasfromYanglingShaanxi.11strain
5、swereisolated,3suspectedstrainswereidentifiedusingmot—phological.physiologicalandbiochemicalcharacteristicsmethods.Afteroptimizingofliquidfermentation,abeststrainb一1wasscreenedwithCPandCFaSstandardand16SrDNAsequencewascarriedout,strainb一1iSconsideredtobeBacillussubtilis,andshows99%homology
6、.Keywords:Bacillussubtilis;screening;indentification枯草芽孢桿菌隸屬于芽孢桿菌科芽孢桿菌屬的細(xì)菌,目前有兩個(gè)亞種,即B.subtilissubsp.subitils(Ehrenberg,cohn,1835,1872)和B.subtilissubsp.spizienii(Nakamuraetal,1999)。革蘭氏陽性,桿狀,單個(gè),大小0.7~o.8弘m×2~3p.m,無莢膜,能運(yùn)動(dòng);芽孢中生或者稍偏,圓柱或者橢圓狀,孢子囊無明顯膨大,孢子大小0.8肚mxl.5~1.8p.m;[收藕日期]2012-09—13[基金項(xiàng)目]國家
7、863項(xiàng)目(2010AAlOZlOl,2011AAl00307,[作者簡介]*[通訊作者]2011ZX08007—002),MATS—BeefCattleSys—tern以及陜西省農(nóng)業(yè)攻關(guān)項(xiàng)目(2010K02—13)資助。白延琴,女,(1985一).陜西子長人,助理畜牧師,子長縣畜牧局工作,主要從事畜牧技術(shù)推廣工作。E-mail:byq521817@126.corn辛亞平(1965一),陜西扶風(fēng)人,博士,副教授,從事養(yǎng)牛學(xué)的教學(xué)科研和推廣工作。E—mail:xin—yaping@126.eom.嚴(yán)格好氧,在營養(yǎng)瓊脂