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1、現(xiàn)代食品科技ModernFoodScienceandTechnology2016,Vol.32,No.9白酒對浙東白鵝肌原纖維蛋白結(jié)構(gòu)的影響11,21111雒宏琳,潘道東,孫楊贏,曹錦軒,吳振,曾小群(1.寧波大學(xué)浙江省動物蛋白食品加工技術(shù)重點實驗室,浙江寧波315211)(2.南京師范大學(xué)食品科學(xué)與營養(yǎng)系,江蘇南京210097)摘要:以浙東大白鵝為原料,拉曼光譜技術(shù)為主要分析手段,研究白酒體積分數(shù)對浙東白鵝胸脯肉肌原纖維蛋白結(jié)構(gòu)的影響。結(jié)果顯示,隨著白酒體積分數(shù)的增大,肌原纖維蛋白溶解度增大(p<0.05),表面疏水
2、性降低(p<0.05)。在白酒體積分數(shù)較低時形成的凝膠特性較好,其微觀結(jié)構(gòu)很均勻,隨著白酒體積分數(shù)的進一步增大,凝膠網(wǎng)絡(luò)孔徑變大,蛋白凝膠保水性顯著降低(p<0.05)。隨-1著白酒體積分數(shù)的增大,二硫鍵的三種構(gòu)象之間發(fā)生了轉(zhuǎn)變;對照組中酰胺Ⅰ帶的主峰出現(xiàn)在1655cm,α-螺旋的含量為40.45%;-1而當(dāng)體積分數(shù)為2%時,1668cm成為主峰(無規(guī)卷曲),α-螺旋和β-折疊含量顯著下降(p<0.05),無規(guī)卷曲顯著上升為31.58%(p<0.05)。結(jié)果表明白酒能夠影響肌原纖維蛋白的結(jié)構(gòu)和凝膠特性。關(guān)鍵詞:浙東白鵝
3、;白酒;肌原纖維蛋白;蛋白結(jié)構(gòu);拉曼光譜文章篇號:1673-9078(2016)9-69-76DOI:10.13982/j.mfst.1673-9078.2016.9.011EffectofDistilledSpiritontheStructuralChangesofMyofibrillarProteinfromZhedongGooseMeat11,21111LUOHong-lin,PANDao-dong,SUNYang-ying,CAOJin-xuan,WUZhen,ZENGXiao-qun(1.KeyLaborat
4、oryofAnimalProteinFoodProcessingTechnologyofZhejiangProvince,NingboUniversity,Ningbo315211,China)(2.FoodScience&NutritionDepartmentofNanjingNormalUniversity,Nanjing210097,China)Abstract:Ramanspectroscopywasappliedtostudythestructuralchangescausedbythevolumefract
5、ionofdistilledspiritonmyofibrillarproteinsextractedfromZhedonggoosebreastmeat.Theresultsindicatedthatincreasingthevolumefractionofthedistilledspiritcausedthesolubilityofmyofibrillarproteintoincrease(p<0.05)andthesurfacehydrophobicitytodecrease(p<0.05).Asevidence
6、dbyscanningelectronmicroscopy,agoodgelpropertyandauniformmicrostructurewereobtainedwhenthevolumefractionofthedistilledspiritwaslow;however,withincreasingvolumefractionofthedistilledspirit,thegelnetworkporesizewasincreasedandthewater-holdingcapacityofproteinswasd
7、ecreasedsignificantly(p<0.05).Theincreaseinthevolumefractionofdistilledspiritcausedconversionsamongthethreeconformationsofthe-1disulfidebond.Forthecontrolgroup,themainamideIRamanbandwasfoundat1655cm,withanα-helixof40.45%.Ata2%volume-1fraction,themainbandwasfound
8、at1668cm(randomcoil),therelativecontentsoftheα-helixandβ-sheetdecreasedsignificantly(p<0.05),andtherandomcoilcontentincreasedsignificantly(34.40%)(p<0.05).Thisindicat