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1、ENTWISLEETAL.:JOURNALOFAOACINTERNATIONALVOL.83,NO.6,20001377FOODCHEMICALCONTAMINANTSLiquidChromatographicMethodwithImmunoaffinityColumnCleanupforDeterminationofOchratoxinAinBarley:CollaborativeStudyA.CATHERINEENTWISLE,ALISONC.WILLIAMS,PETERJ.MANN,andPHILIPT.SLACKLeatherheadFo
2、odRA,RandallsRd,Leatherhead,SurreyKT227RY,UKJOHNGILBERTMinistryofAgriculture,FisheriesandFood,CentralScienceLaboratory,SandHutton,YorkYO411LZ,UKCollaborators:P.Burdaspal;E.Eklund;J.Gardikis;B.Hald;M.-P.Herry;K.J?rgensen;H.Kandler;R.Maas;M.-L.Martins;S.Patel;M.Schuster;M.Solfr
3、izzo;E.Strassmeir;R.Tiebach;T.TrogersenAcollaborativestudywasconductedtoevaluateaHORRATvalues,atthelowlevelofdeterminationliquidchromatographic(LC)methodwithforochratoxinAinbarley.immunoaffinitycolumncleanupfordeterminationofochratoxinA.Themethodwastestedat3con-centrationleve
4、lsofochratoxinAinbarley,whichithimprovementsinmethodsofanalysisforrepresentpossiblefutureEuropeanregulatorylim-ochratoxinA(particularlylimitsofdetection),andits.ThetestportionwasextractedwithWtheirwideapplicationindietarysurveys,acetonitrile–waterbyblendingathighspeed.Theochr
5、atoxinAhasbeenfoundinanincreasinglywiderangeofextractwasfiltered,dilutedwithphosphate-differentfoodstuffs.Oneofthemaincontributingfactorstobufferedsaline(PBS),andappliedtoanthisimprovementinmethodologyhasbeentheintroductionofochratoxinAimmunoaffinitycolumn.Thecolumnmonoclonal
6、antibodiesusedinimmunoaffinitycolumns(1).waswashedwithwaterandtheochratoxinAPreviously,analyticalmethodsforochratoxinAhaveusedelutedwithmethanol.Thesolventwasthenevapo-solventextractionfollowedbyeitherliquid–liquidextractionratedandtheresidueredissolvedininjectionsol-vent.Aft
7、erinjectionofthissolutionontore-orsolid-phaseextraction(SPE)cleanup(eitherC18orsilica)coupledwithachoiceofdetectionmethodssuchasen-versed-phaseLCcolumn,ochratoxinAwaszyme-linkedimmunosorbentassay(ELISA),thinlayerchro-measuredbyfluorescencedetection.Eightsam-matography(TLC),or
8、liquidchromatography(LC).Theuseplesoflowlevelnaturallycontaminatedba
