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《冠狀病毒Coronavirusl論文-1992 A Serologic Investigation for Coronavirus and Breda Virus Antibody in Winter Dysentery of Dairy Cattle in the North.pdf》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、JVetDiagnInvest4:450-452(1992)AserologicinvestigationforcoronavirusandBredavirusantibodyinwinterdysenteryofdairycattleinthenortheasternUnitedStatesH.J.VanKruiningen,V.P.Castellano,M.Koopmans,L.L.Harris9Since1980,theamountofevidenceimplicatingabovinefectedcalf.BRV2is
2、usedforroutinediagnostictestsbecause8coronavirusasthecauseofwinterdysenteryhasbeenin-itismorestablethanBRVl.AdirectblockingELISAthat13creasing.Japaneseworkerswerethefirsttorecoveraco-hasbeenpreviouslydescribedwasusedtodetectantibody159,11ronavirusfromthefecesofacoww
3、ith“epizooticdiarrhea”;toBredavirus.2thisdiscoverywasfollowedbysimilarreportsfromBelgiumSerumneutralizationanti-coronavirusantibodytiterswere1andtheUnitedStates.Becausecoronavirus-likeparticlesdeterminedbyamethodsimilartothatemployedforrota-6andcoronavirusantigencan
4、befoundinthefecesofahighviruses.Nebraskastraincalfhooddiarrheacoronavirus(No.proportionofnormaldairycowsinsomeherdsduringthe874fromtheAmericantypeculturecollection)wasgrown3,4winterstablingseason,thesignificanceoftheseisolationsinMadinDarbybovinekidneycellsinthepres
5、enceof0.1%hasbeeninterpretedwithsomereservation.Serologicstudiespancreatinandintheabsenceofserum.Four-foldserialdi-50revealedhemagglutination-inhibitionseroconversiontoref-lutionsoftestserawerechallengedwith200-500TCIDforerencestrainsofbovinecoronavirusin59%ofaffect
6、edJap-90minutesat37C.Four-or5-day-oldcellmonolayersin15anesecattle;workersfromOhioreported4-foldorgreatermicroplatewells(growninEagle’sminimumessentialme-risesinserumneutralization(SN)titersin19of26animalsdium[EMEM]with10%fetalcalfserum)werewashedfree14(73%);andtheB
7、ritish,usingalatexagglutinationinhibitionofserum,inoculatedwith200μ1oftheserum-virusmixturestest,foundseroconversionin3of5affectedcattle?Were-(adsorbedat37Cfor120minutes),thenrinsedandincubatedcentlyreported63%seroconversionbyanenzyme-linkedfurther(withEMEMandpancre
8、atin).Thetestwasreadafterimmunosorbentassay(ELISA)method,in35sickanimals2-4days,andtheantibodytiterwasexpressedastherecip-16from8herdswith
