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1、656aWednesday,February29,2012ethanolamine(PE18:1/16:0)andphosphatidylserine(PS18:0/18:1).Sincethis3332-PosBoardB193membraneisthickerthantheestimatedlengthoftolaasinchannel,mismatchinElectricalAspectsofMembranePermeabilizationbyNewPolycationicthicknessmaymakethechannelunstable.Ph
2、ospholipidscomposedofmediumPeptidesDerivedfromtheCry11BbProtoxinorshort-chainfattyacidsmaybehelpfultothestabilityoftolaasinchannelbyVictorV.Lemeshko,JoseA.Alvarez.makingthemembranethinner.WhenphosphatidylethanolaminesmadewithNationalUniversityofColombia,Medellin,Colombia.decanoi
3、cacids(capricacid,DDPE),myristicacids(DMPE),andstearicacidsThepermeabilizationofmitochondrialandplasmamembranesbysynthetic(DSPE)wereadded,DDPE(200nM)facilitatedtolaasin-inducedhemolysis.polycationicpeptidesderivedfromtheCry11Bbprotoxinwasstudied.TheWhentheconcentrationofDDPEwasa
4、djustedfrom0.2-200nM,thehemoly-peptidesweredesignedwiththeaimtofurtherstudyoftheirantimicrobialsiswasstimulatedattheconcentrationsabove2nM.KsvalueofDDPEeffectandanticanceractivities.Itwasobservedthatthemembranepermeabilizingwasobtainedat6mMDDPE.Whenthepreincubationeffectoftolaas
5、inandactivityofthesepolycationicpeptidesstronglyincreasedbythetransmem-DDPEwasmeasured,bindingoftolaasintoDDPEwascompletedwithin5branepotential(minusinside).Thisphenomenonwascon?rmedbythestudymin.Inthelipidbilayerrecording,theadditionofDDPEincreasedtolaasinofthearti?cialplanarme
6、mbranepermeabilization:applying50mVtothepla-channelactivitybyincreasingopenprobability.Therefore,tolaasinmoleculesnarmembrane(minustothetransside)during30secinducedtime-dependentmakemorestablechannelswithphospholipidscomposedofmedium-chainincreaseinthetransmembranecurrentinthepr
7、esenceofapeptideaddedtofattyacids.thecisside,whilesubsequentapplicationoftheoppositepotentialcauseditsdecrease.Wealsoobservedthattheactivationofthecellsuicidemecha-3330-PosBoardB191nism,whichpartiallyrevealedinphosphatidylserineexposureatthecellsur-2DCompetitionEffectofDDPEandZn
8、ontheHemolysisInducedbyface,signi?cantlyincreas
