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1、HemadsorptionbyCoronavirusStrainOC43(36105)ALBERTZ.KAPIKIAN,HARVEYD.JAMES,JR.,SARAJ.KELLY,LULAM.KING,ANNIEL.VAUGHN,ANDROBERTM.CHANOCKLaboratoryofInfectiousDiseases,NationalInstituteofAllergyandInfectiousDiseases,NationalZnstitutesofHealth,DHEW,Bethesda,Maryland20014Coronavirusesappeartobeimportante
2、tio-nancemediumconsistingofequalpartsoflogicagentsofadultupperrespiratorytractEagle'sminimumessentialmediuminEarle'sillnesses(1-3).However,studyofthesevi-BSSandmedium199,100unitsofpenicil-ruseshasbeenhamperedbythelackofcon-lin/mland100mgofstreptomycin/ml.venientandsuitablemethodsfortheirpropa-Serum
3、wasnotusedinthemaintenancegation.Nineofthe23coronavirusstrainsmedium.recoveredtodatewereoriginallyisolatedinInfectivitytitrations.Tenfolddilutionsofhumanembryonictracheal(HET)organcul-virusweremadeinHanks'BSScontainingturesonly,whereastheremaining14,allof0.5%gelatin,100unitsofpenicillinJm1andwhichw
4、eresimilartoaprototypestrain100,pgstreptomycin/ml.Two-tenthsmilliliterdesignated2293,wereisolatedwithdifficultyoftheappropriatevirusdilutionswasinocu-inmonolayertissuecultures(2,4-9).latedintoeachtubeculture;theculturesB814virus,thefirstorganculture(OC)wereexaminedforhemadsorptionasde-strain,twooth
5、erOCstrains,LPandEVS,scribedbelowatvaryingintervalsundertheandstrain2293weresubsequentlyrecoveredlowpowerofalightmicroscope.IfculturesinL132cellmonolayersfromoriginalnasalweretobeexaminedagainforhemadsorptionwashingsobtainedfromvolunteersinoculatedatalaterdate,themaintenancemediumcon-withtheseagent
6、s(8,10).Morerecently,tainingredbloodcellswasdecantedandtheOC43virus(andOC38virus,anidenticalmonolayerwaswashedtwicewithHanks'BSSagent)bothofwhichwereoriginallyrecov-beforetheadditionofnewmaintenanceeredinOCandlateradaptedtogrowinmedium.sucklingmice,werenotonlyshowntodirect-Serology.Allserawereinact
7、ivatedat56"lyhemagglutinatevariouserythrocytes,butfor30min.Intheneutralizationtest,equalwerealsoadaptedtogrowinmonolayermon-volumesofvirusandtheappropriateserumkeykidneycellcultures(11-13).Stimulateddilutio
