冠狀病毒Coronavirusl論文-2016 [Springer Protocols Handbooks] Animal Coronaviruses __ Feline Coronavirus RT-PCR Assays for Feline Infectious Perit.pdf

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1、Chapter15FelineCoronavirusRT-PCRAssaysforFelineInfectiousPeritonitisDiagnosisTakehisaSomaAbstractFelineinfectiousperitonitis(FIP)isahighlyfatalsystemicdiseaseincats,causedbyfelinecoronavirus(FCoV)infection.FCoVusuallyhaslittleclinicalsigniTcance;however,amutati

2、onofthisavirulentvirus(felineentericcoronavirus)toavirulenttype(FIPvirus)canleadtoFIPincidence.ItisdifTculttodiag-noseFIP,sincethevirusescannotbedistinguishedusingserologicalorvirologicalmethods.Recently,genetictechniques,suchasRT-PCR,havebeenconductedforFIPdia

3、gnosis.Inthischapter,thereliabilityofRT-PCRandproceduresusedtodetermineFCoVinfectionaspartofantemortemFIPdiagnosisisdescribed.KeywordsDiagnosis,Felinecoronavirus,Felineinfectiousperitonitis,RT-PCR1IntroductionFelineinfectiousperitonitis(FIP)isanimmune-mediatedp

4、rogres-siveandsystemicinfectiousdiseaseoccurringindomesticcatsandwildfelids,andcausedbyinfectionwithfelinecoronavirus(FCoV),asingle-strandedRNAvirus,whichhasbeenclassiTedasAlphacoronavirusalongwithcaninecoronavirus(CCoV)andtransmissiblegastroenteritisvirus[1,2]

5、.FCoVistransmittedbythefecal-oralrouteandusuallycausesamildtoinapparententeritis[2].FIPisconsideredtobeinducedbyavirulentmutant(FIPvirus;FIPV)ofthisentericFCoV(felineentericcoronavirus;FECV)[2,3].TheincidenceofFIPisgenerallyaslowas1D3%inFCoV-infectedcats,though

6、itvariesdependingonage,breed,environment,andsuperinfectionwithotherviruses[2,4D6].Itisdividedintotwobasicclinicalforms,effusiveFIP,inwhicheffusionisobservedinthebodycavity,andnon-effusiveFIP,inwhichmultiplepyogranulomalesionsareobserved,thoughdiffer-encesinlesi

7、onsarein?uencedbyindividualimmunity[7].Furthermore,therearetwotypes(IandII)ofFCoV,withFCoVtypeIIconsideredtoarisebyarecombinationofFCoVtypeIandLeyiWang(ed.),AnimalCoronaviruses,SpringerProtocolsHandbooks,DOI10.1007/978-1-4939-3414-0_15,?SpringerScience+Business

8、MediaNewYork2016161162TakehisaSomaCCoV[8D10].Basedongeneticandserologicalinvestigations,FCoVtypeIisoverwhelminglydominantascomparedtotypeIIandmixedinfectionwithbothtypesisno