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1、AdvancePublicationTheJournalofVeterinaryMedicalScienceAcceptedDate:6May2018J-STAGEAdvancePublishedDate:18May20181Virology2NOTE3Along-termanimalexperimentindicatingpersistentinfectionofbovine4coronavirusincattle51),1)1,2)1,3)6ToruKanno*,RyokoIshihara,Shinichi
2、Hatama,IkuoUchida781)DivisionofPathologyandPathophysiology,NationalInstituteofAnimalHealth,49Hitsujigaoka,Toyohira,Sapporo,Hokkaido062-0045,Japan102)Currentaddress:DivisionofViralDiseaseandEpidemiology,NationalInstituteof11AnimalHealth,3-1-5Kannondai,Tsukuba
3、,Ibaraki305-0856,Japan123)Currentaddress:DivisionofBacterialandParasiticDisease,NationalInstituteof13AnimalHealth,3-1-5Kannondai,Tsukuba,Ibaraki305-0856,Japan14*Correspondenceto:ToruKanno15DairyHygieneUnit,DivisionofPathologyandPathophysiology,NationalInstit
4、uteof16AnimalHealth,4Hitsujigaoka,Toyohira,Sapporo,Hokkaido062-0045,Japan17Tel:+81-11-851-5226;Fax:+81-11-853-0767;E-mail:kannot@affrc.go.jp18Runninghead:PERSISTENTINFECTIONOFBCoVINCATTLE1920121Abstract22Along-termanimalexperimentinvolvinginoculationwithbovi
5、necoronavirus(BCoV)23wasconductedtoverifyitspersistentinfectionincattle.Threecolostrum-deprived24Holsteincalveswerehousedseparatelyinindividualroomsofahigh-containment25facilityandinoculatedwiththeBCoVstrainKumamoto/1/07.Untiltheendofthe26experiment(1,085,70
6、0,and280days,respectively),viralRNAsweredetected27sporadicallybyRT-PCRandnestedPCRfromplasma,nasaldischarge,andfeces.28Seroconversionandtiterchangeswerevalidatedbyhemagglutinationinhibitiontests29andneutralizationtests.Amongthesamples,nasaldischargeshowedahi
7、gherviral30positivitythanfeces,whichseemedtobeassociatedwithpositivedetectioninthe31plasma.ThesedatademonstratetheexistenceofpersistentinfectionofBCoVinthe32respiratorytissuesofcattle.3334Keywords:bovinecoronavirus,long-termanimalexperiment,persistentinfecti
8、on3536237Bovinecoronavirus(BCoV)isamemberoftheorderNidovirales,family38Coronaviridae,subfamilyCoronavirinae,genusBetacoronavirus,species39Betacoronavirus-1.ThisspeciesalsoincludesHumancoronaviru
