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《尼古丁論文：尼古丁降解菌的篩選鑒定及降解特性的研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、尼古丁論文：尼古丁降解菌的篩選鑒定及降解特性的研究【中文摘要】本文利用富集篩選的方法從杭州農(nóng)藥廠的活性污泥中分離得到兩株尼古丁高效降解菌,分別命名為HZN1和TW5,經(jīng)過對(duì)這兩株菌的生理生化特性、形態(tài)特征和16SrDNA序列同源性分析,將其分別鑒定為申氏桿菌屬(Shinellasp.)和假單胞屬(Pseudomonassp.)。研究表明,菌株HZN1和TW5都能以尼古丁為唯一碳源、氮源和能源生長(zhǎng),能在質(zhì)量濃度為4.0g/L的尼古丁基礎(chǔ)鹽培養(yǎng)基中生長(zhǎng),菌株生長(zhǎng)和降解尼古丁的最適溫度均為30℃,最適pH值為6.5~7.5。菌株HZN1分別能在4h、9h、12h內(nèi)
2、將初始濃度分別為0.2、0.5、1.0g/L的尼古丁降解接近完全,而外加碳源葡萄糖會(huì)減緩HZN1降解尼古丁的速率;溫度和pH值對(duì)HZN1的降解效率影響不大;菌株TW5分別能在6h和9h內(nèi)將初始濃度分別為0.2和0.5g/L的尼古丁降解接近完全,當(dāng)尼古丁質(zhì)量濃度上升為1.5g/L時(shí),降解效率受到明顯影響,12h內(nèi)只能降解70%的尼古丁,溫度和pH值對(duì)TW5的降解效率影響較大。進(jìn)一步利用氣質(zhì)聯(lián)用儀(GC-MS)研究了菌株HZN1降解尼古丁的代謝產(chǎn)物,根據(jù)檢測(cè)到的三個(gè)產(chǎn)物:麥思明、可鐵林和尼古提林,推測(cè)了菌株HZN1在降解尼古丁過程中可能存在的降解途徑。菌株HZN
3、1和TW5具有在惡劣環(huán)境中處理高濃度尼古丁廢水的潛力,其中HZN1所屬的申氏桿菌屬(Shinellasp.)還未見報(bào)道有能夠降解尼古丁的菌種,我們的研究為生物降解尼古丁提供了新的依據(jù)?！居⑽恼縏henicotine-degradingbacteriumHZN1andTW5wereisolatedfromactivatedsludgeofHangzhoupesticidefactoryandidentifiedasShinellasp.andPseudomonassp.basedontheirphysiologicalcharacteristicsanda
4、nalysisof16SrDNAgene,respectively.StrainHZN1andTW5arecapableofusingnicotineasthesolesourceofcarbon,nitrogenandenergy,andcangrowthonthemineralsaltsmediumwithnicotineconcentration4.0g/L.TheoptimumtemperatureandpHforstrainHZN1andTW5growthandnicotinedegradationwere30℃and7.0,respectivel
5、y.StrainHZN1coulddegradeapproximately100%of0.2,0.51.0g/Lofnicotinewithin4,9,12h,respectively;thedegradationofnicotinewasslightlyslowerwiththepresenceofglucose;strainHZN1coulddegradenicotineefficientlyinarelativelybroadrangeoftemperature(23,30,37℃)andpHvalues(5.5~9.0).StrainTW5could
6、degradeapproximately100%of0.2,0.5g/Lofnicotinewithin6,9h,respectively;itcouldonlydegrade70%of1.5g/Lofnicotineat12h;temperatureandpHhavehigheffectsonnicotinedegradationbystrainTW5.ThreeintermediatemetaboliteswereproducedbythestainHZN1andidentifiedascotinine,myosmineandnicotyrineusin
7、ggaschromatography-massspectrometry(GC-MS).Andpossiblenicotine-degradingpathwayofstrainHZN1waspresumed.TheseresultsindicatethatthestrainHZN1andTW5couldpotentiallybeusefulfortheremediationofnicotinepollutioninmal-condition.Thisisthefirstreportofnicotine-degradingstrainfromthegenusof
8、Shinella.Ourfindingswouldp
